Anticorps Polyclonal de lapin anti-POLQ

POLQ Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28590-1-PBS

Synonymes

POLH, EC:2.7.7.49, DNA polymerase theta, DNA polymerase eta

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Galerie de données de validation

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  WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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  WB analysis using 28590-1-AP (same clone as 28590-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28590-1-AP (POLQ antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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  IF Staining of A375 using 28590-1-AP (same clone as 28590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A375 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 28590-1-AP (same clone as 28590-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLQ antibody (28590-1-AP) at dilution of 1:600 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red). This data was developed using the same antibody clone with 28590-1-PBS in a different storage buffer formulation.

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Informations sur le produit

28590-1-PBS cible POLQ dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLQ Protéine recombinante Ag29643
• Nom complet: polymerase (DNA directed), theta
• Masse moléculaire calculée: 290 kDa
• Poids moléculaire observé: 250~290 kDa, 197 kDa
• Numéro d’acquisition GenBank: NM_199420
• Symbole du gène: POLQ
• Identification du gène (NCBI): 10721
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

DNA Polymerase Theta (POLQ) is a DNA polymerase involved in error-prone translesion DNA synthesis (TLS) and error-prone repair of DNA double-strand breaks (DSBs). The enzyme has several unique properties, including low fidelity and the ability to insert and extend past abasic sites and thymine glycol lesions. Accumulating evidence showed that POLQ participates in the development and progression of human cancers because of its critical functions in the repair of genomic double-strand breaks (PMID: 34517891, 27264557). The POLQ cDNA encodes a polypeptide of 2592 amino acids with a predicted mass of 290 kDa, but POLQ is readily proteolyzed with smaller bands at approximately 70 kDa and 110 kDa (PMID: 17334634, 14576298).

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