Anticorps Polyclonal de lapin anti-POLR1A

POLR1A Polyclonal Antibody for WB, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20595-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis of HeLa using 20595-1-AP (same clone as 20595-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

• 

  WB analysis using 20595-1-AP (same clone as 20595-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HepG2 using 20595-1-AP (same clone as 20595-1-PBS)

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20595-1-AP (POLR1A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

• 

  IP experiment of HeLa using 20595-1-AP (same clone as 20595-1-PBS)

  IP result of anti-POLR1A (IP:20595-1-AP, 4ug; Detection:20595-1-AP 1:300) with HeLa cells lysate 2400ug. This data was developed using the same antibody clone with 20595-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20595-1-PBS cible POLR1A dans les applications de WB, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR1A Protéine recombinante Ag14543
• Nom complet: polymerase (RNA) I polypeptide A, 194kDa
• Masse moléculaire calculée: 1720 aa, 195 kDa
• Poids moléculaire observé: 190-195 kDa
• Numéro d’acquisition GenBank: BC017115
• Symbole du gène: POLR1A
• Identification du gène (NCBI): 25885
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

POLR1A, DNA-directed RNA polymerase I subunit RPA1, is a DNA-dependent RNA polymerase catalyzes the transcription of DNA into RNA. RNA polymerase I (Pol I) located in the nucleolus and transcribes class I genes, which code for large ribosomal RNA. Different subunits of the Pol I transcription are targets of various physiological stimuli, which suggests that multiple signaling pathways are involved in carrying out Pol I ranscription. Together with the second largest subunit, it is the catalytic core component of RNA polymerase I that synthesizes ribosomal RNA precursors.

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