Anticorps Polyclonal de lapin anti-POLR2A

• Phare
• Validé par KD/KO

POLR2A Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, singe, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(6)

Avis (1)

N° de cat : 20655-1-AP

Synonymes

hRPB220, hsRPB1, Pol II, POLR2, POLR2A, POLRA, RNA polymerase II subunit B1, RPB1, RPBh1, RpIILS, RPO2, RPOL2

---

Galerie de données de validation

• 

  WB analysis of HeLa using 20655-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 20655-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HepG2 using 20655-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 20655-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20655-1-AP (POLR2A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human pancreas cancer using 20655-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer tissue slide using 20655-1-AP (POLR2A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse pancreas using 20655-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 20655-1-AP (POLR2A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du pancréas humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IF: See 2 publications below

Informations sur le produit

20655-1-AP cible POLR2A dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, singe, souris

• Réactivité: Humain, singe, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: polymerase (RNA) II (DNA directed) polypeptide A, 220kDa
• Masse moléculaire calculée: 217 kDa
• Poids moléculaire observé: 210-250 kDa
• Numéro d’acquisition GenBank: NM_000937
• Symbole du gène: POLR2A
• Identification du gène (NCBI): 5430
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Front Cell Dev Biol; Brahma-Related Gene 1 Deficiency in Endothelial Cells Ameliorates Vascular Inflammatory Responses in Mice.; Authors - Yuanyuan Zhang
human	WB,IF	J Exp Clin Cancer Res; LncMAPK6 drives MAPK6 expression and liver TIC self-renewal.; Authors - Guanqun Huang
human	WB	Cell Death Discov; Cezanne promoted autophagy through PIK3C3 stabilization and PIK3C2A transcription in lung adenocarcinoma; Authors - Yadong Wang ; KD Validated
human	WB	Cell Rep; NEIL1 drives the initiation of colorectal cancer through transcriptional regulation of COL17A1; Authors - Jing-Hua Cao
human	WB	Nat Commun; Thermal proteome profiling reveals fructose-1,6-bisphosphate as a phosphate donor to activate phosphoglycerate mutase 1; Authors - Yanling Zhang
rat	WB,IF	Neuropharmacology; Neurotransmitter Imbalance and Amygdala Synaptic Plasticity in Lumbar Disc Herniation-induced Chronic Pain and Related Emotional Disturbances:A multi-omics analysis; Authors - Zhenyu Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

---

FH

Marta (Verified Customer) (02-28-2022)

Method: dot blot

• Applications: Other
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: glioma

POLR2A Antibody Other validation (1:1000 dilution) in glioma (Cat no:20655-1-AP)

{{ptg:RelatedPrimaryAntibodies}}