Anticorps Polyclonal de lapin anti-POLR2I

• Phare
• Validé par KD/KO

POLR2I Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 17270-1-PBS

Synonymes

DNA-directed RNA polymerase II subunit I, DNA-directed RNA polymerase II subunit RPB9, hRPB14.5, RPB14.5, RPB9

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Galerie de données de validation

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  WB analysis using 17270-1-AP (same clone as 17270-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 17270-1-AP (POLR2I antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse stomach using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse stomach tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IHC staining of rat liver using 17270-1-AP (same clone as 17270-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat liver tissue slide using 17270-1-AP (POLR2I antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 17270-1-AP (same clone as 17270-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POLR2I antibody (17270-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red). This data was developed using the same antibody clone with 17270-1-PBS in a different storage buffer formulation.

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Informations sur le produit

17270-1-PBS cible POLR2I dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR2I Protéine recombinante Ag11027
• Nom complet: polymerase (RNA) II (DNA directed) polypeptide I, 14.5kDa
• Masse moléculaire calculée: 125 aa, 15 kDa
• Poids moléculaire observé: 15 kDa
• Numéro d’acquisition GenBank: BC017112
• Symbole du gène: POLR2I
• Identification du gène (NCBI): 5438
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

POLR2I, also known as RPB9 or hRPB14.5, is a small core subunit of RNA polymerase II involved in DNA repair and many transcription-related processes (PMID: 21450810). It is expressed in many tissues and may play a key role in the pathological process of hypertensive nephropathy (PMID: 34422001).

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