Anticorps Polyclonal de lapin anti-POLR3A

POLR3A Polyclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 28842-1-PBS

Synonymes

RNA polymerase III subunit C1, hRPC155, EC:2.7.7.6, DNA-directed RNA polymerase III subunit RPC1, DNA-directed RNA polymerase III subunit A

---

Galerie de données de validation

• 

  WB analysis using 28842-1-AP (same clone as 28842-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 28842-1-AP (POLR3A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 28842-1-PBS in a different storage buffer formulation.

• 

  IF Staining of A431 using 28842-1-AP (same clone as 28842-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using POLR3A antibody (28842-1-AP) at dilution of 1:400 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). This data was developed using the same antibody clone with 28842-1-PBS in a different storage buffer formulation.

---

Informations sur le produit

28842-1-PBS cible POLR3A dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POLR3A Protéine recombinante Ag29036
• Nom complet: polymerase (RNA) III (DNA directed) polypeptide A, 155kDa
• Masse moléculaire calculée: 1390 aa, 156 kDa
• Poids moléculaire observé: 155 kDa
• Numéro d’acquisition GenBank: BC041089
• Symbole du gène: POLR3A
• Identification du gène (NCBI): 11128
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

POLR3A encodes the largest subunit of human RNA polymerase III (RPC1), which contributes to the catalytic activity of Pol III and is part of the active centre of the polymerase. Pol III is responsible for the transcription of untranslated RNAs (PMID: 25339210). Bi-allelic missense variants in POLR3A have been associated with phenotypes distinct from WRS: hypogonadotropic hypogonadism and hypomyelinating leukodystrophy with or without oligodontia (PMID: 30414627).

{{ptg:RelatedPrimaryAntibodies}}