Anticorps Polyclonal de lapin anti-POM121

• Phare
• Validé par KD/KO

POM121 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 15645-1-PBS

Synonymes

POM121A, NUP121, Nucleoporin Nup121, Nuclear envelope pore membrane protein POM 121A, Nuclear envelope pore membrane protein POM 121

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Galerie de données de validation

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  WB analysis using 15645-1-AP (same clone as 15645-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 15645-1-AP (POM121 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 15645-1-AP (same clone as 15645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon using 15645-1-AP (same clone as 15645-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon tissue slide using 15645-1-AP (POM121 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

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  IF Staining of mouse brain using 15645-1-AP (same clone as 15645-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 15645-1-AP (POM121 antibody) at dilution of 1:200 and Alexa Fluor 488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

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  IF Staining of HCT 116 using 15645-1-AP (same clone as 15645-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HCT 116 cells using POM121 antibody (15645-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red). This data was developed using the same antibody clone with 15645-1-PBS in a different storage buffer formulation.

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Informations sur le produit

15645-1-PBS cible POM121 dans les applications de WB, IHC, IF/ICC, IF-P, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POM121 Protéine recombinante Ag8177
• Nom complet: POM121 membrane glycoprotein (rat)
• Masse moléculaire calculée: 984 aa, 99 kDa
• Poids moléculaire observé: 140-150 kDa
• Numéro d’acquisition GenBank: BC008794
• Symbole du gène: POM121
• Identification du gène (NCBI): 9883
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

POM121 is an integral membrane protein that localizes to the central spoke ring complex and participates in anchoring the nuclear pore complex to the nuclear envelope. Catalog#15645-1-AP do well in identifying the nuclear envelope in immunofluorescence experiments.

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