Anticorps Monoclonal anti-POMC

POMC Monoclonal Antibody for IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, souris

Applications

IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

3C12C7

N° de cat : 66358-1-PBS

Synonymes

3C12C7, ACTH, ACTH Melanotropin alpha, Adrenocorticotropic hormone, Beta LPH Lipotropin gamma

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Galerie de données de validation

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  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:2000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IHC staining of human pituitary using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pituitary tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 10x lens). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse pancreas using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 66358-1-Ig (POMC antibody) at dilution of 1:200 (under 40x lens). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using POMC antibody (66358-1-Ig, Clone: 3C12C7 ) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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  FC experiment of HeLa using 66358-1-Ig (same clone as 66358-1-PBS)

  1X10^6 HeLa cells were intracellularly stained with 0.2 ug Anti-Human POMC (66358-1-Ig, Clone:3C12C7) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (green), and 0.2 ug Mouse IgG1 Isotype Control (66360-1-Ig, Clone: T1F8D3F10) (black). Cells were fixed with 4% PFA and permeabilized with 0.1% TritonX-100. This data was developed using the same antibody clone with 66358-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66358-1-PBS cible POMC dans les applications de IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: POMC Protéine recombinante Ag17311
• Nom complet: proopiomelanocortin
• Masse moléculaire calculée: 267 aa, 29 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC065832
• Symbole du gène: POMC
• Identification du gène (NCBI): 5443
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pro-opiomelanocortin (POMC) is a prohormone that encodes multiple smaller peptide hormones within its structure. POMC plays important roles in many pathological and physiological processes, including regulating energy metabolism, immune reactions, and sexual functions. POMC expression differs quantitatively and qualitatively among most normal tissues. The large molecule of POMC is the source of several important biologically active substances. POMC can be cleaved enzymatically into the following peptides: d NPP, γ-MSH, ACTH, α-MSH, CLIP, Lipotropin β, Lipotropin γ, β-MSH, β endorphin and Met-enkephalin.

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