Anticorps Recombinant de lapin anti-POMP

• Phare
• Validé par KD/KO

POMP Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

240563D7

N° de cat : 83607-6-RR

Synonymes

PNAS 110, hUMP1, HSPC036, HSPC014, C13orf12

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Galerie de données de validation

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  WB analysis using 83607-6-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83607-6-RR (POMP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat skin using 83607-6-RR

  rat skin tissue were subjected to SDS PAGE followed by western blot with 83607-6-RR (POMP antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 83607-6-RR

  WB result of POMP antibody (83607-6-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POMP transfected HepG2 cells.

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  Affinity and Kinetic Characterization of 83607-6-RR

  Biolayer interferometry (BLl) kinetic assays of 83607-6-RR against Human POMP were performed. The affinity constant is 0.424 nM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2, cellules HL-60, cellules Jurkat, cellules L02, cellules SW480, tissu cutané de rat

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83607-6-RR cible POMP dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: POMP Protéine recombinante Ag6987
• Nom complet: proteasome maturation protein
• Masse moléculaire calculée: 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC003390
• Symbole du gène: POMP
• Identification du gène (NCBI): 51371
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Proteasome maturation protein(POMP), is an essential factor of mammalian proteasome biogenesis. It facilitates the main steps in 20S core complex formation at the ER to coordinate the assembly process and to provide cells with freshly formed proteasomes at their site of function. A single nucleotide deletion in this gene causes an autosomal recessive skin disorder, keratosis linearis with ichthyosis congenita and sclerosing keratoderma (KLICK) syndrome.

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