Anticorps Polyclonal de lapin anti-PON1

PON1 Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

N° de cat : 18155-1-AP

Synonymes

A esterase 1, Aromatic esterase 1, ESA, K 45, paraoxonase 1, PON, PON 1, PON1, Serum aryldialkylphosphatase 1

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Galerie de données de validation

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  WB analysis of human plasma using 18155-1-AP

  human blood were subjected to SDS PAGE followed by western blot with 18155-1-AP (PON1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse liver using 18155-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 18155-1-AP (PON1 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse liver using 18155-1-AP

  IP Result of anti-PON1 (IP:18155-1-AP, 4ug; Detection:18155-1-AP 1:500) with mouse liver tissue lysate 4000ug.

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  IHC staining of human liver using 18155-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18155-1-AP (PON1 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver using 18155-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 18155-1-AP (PON1 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: plasma humain, tissu hépatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IHC: See 2 publications below
• IF: See 2 publications below
• IP: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

18155-1-AP cible PON1 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PON1 Protéine recombinante Ag12934
• Nom complet: paraoxonase 1
• Masse moléculaire calculée: 355 aa, 40 kDa
• Poids moléculaire observé: 35 kDa, 40-45 kDa
• Numéro d’acquisition GenBank: BC074719
• Symbole du gène: PON1
• Identification du gène (NCBI): 5444
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PON1, also named PON and K-45, belongs to the paraoxonase family. PON1 hydrolyzes the toxic metabolites of a variety of organophosphorus insecticides. It is capable of hydrolyzing a broad spectrum of organophosphate substrates and several aromatic carboxylic acid esters. PON1 may mediate enzymatic protection of low-density lipoproteins against oxidative modification and the consequent series of events leading to atheroma formation. PON1 was identified as a triple band in a range of approximately 35-40 kDa. For glycosylated, the MW of PON1 is migrated 42-45kd, while the 35 kDa band represents the unglycosylated form (PMID: 17906223, 32002976, 21852972). The antibody has cross-reaction to PON2 and PON3.

Publications

Species	Application	Title
human	WB	Cell Death Dis; GPx1 is involved in the induction of protective autophagy in pancreatic cancer cells in response to glucose deprivation.; Authors - Qingcai Meng
human	IF	Front Cell Dev Biol; Significance of Liver Zonation in Hepatocellular Carcinoma.; Authors - Shizhe Yu
mouse	WB,IP	Cell Mol Neurobiol; PGC-1α Inhibits Schwann Cell Dedifferentiation and Delays Peripheral Nerve Degeneration by Targeting PON1; Authors - Ying Zou
mouse	WB,IF	Biomed Pharmacother; GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes; Authors - Xueqi Chen
human,mouse	IHC,ELISA	Elife; Multi-targeted therapy resistance via drug-induced secretome fucosylation; Authors - Mark Borris D Aldonza
mouse	IHC	Biochim Biophys Acta Mol Cell Biol Lipids; Paraoxonase 1 in endothelial cells impairs vasodilation induced by arachidonic acid lactone metabolite.; Authors - Dan Gilad