Anticorps Polyclonal de lapin anti-PON2

PON2 Polyclonal Antibody for FC, IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14379-1-AP

Synonymes

A esterase 2, Aromatic esterase 2, paraoxonase 2, PON 2, PON2, Serum aryldialkylphosphatase 2

---

Galerie de données de validation

• 

  WB analysis of human liver using 14379-1-AP

  human liver tissue were subjected to SDS PAGE followed by western blot with 14379-1-AP (PON2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 14379-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 14379-1-AP (PON2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  IP experiment of L02 using 14379-1-AP

  IP Result of anti-PON2 (IP:14379-1-AP, 4ug; Detection:14379-1-AP 1:500) with L02 cells lysate 3200ug.

• 

  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14379-1-AP (PON2 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 14379-1-AP (PON2 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14379-1-AP (PON2 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 14379-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer using 14379-1-AP (PON2 antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 14379-1-AP

  Immunofluorescent analysis of HepG2 cells, using PON2 antibody 14379-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

• 

  FC experiment of HepG2 using 14379-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug PON2 antibody (14379-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

---

Applications testées

• Résultats positifs en WB: tissu hépatique humain, cellules L02
• Résultats positifs en IP: cellules L02
• Résultats positifs en IHC: tissu de cancer du foie humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

14379-1-AP cible PON2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PON2 Protéine recombinante Ag5759
• Nom complet: paraoxonase 2
• Masse moléculaire calculée: 39 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: BC046160
• Symbole du gène: PON2
• Identification du gène (NCBI): 5445
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PON2(Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).

Publications

Species	Application	Title
human	IHC	FASEB J; Breaking the chain at the membrane: paraoxonase 2 counteracts lipid peroxidation at the plasma membrane.; Authors - Henning Hagmann
mouse	WB	Am J Pathol; Expression of myocilin mutants sensitizes cells to oxidative stress-induced apoptosis: implication for glaucoma pathogenesis.; Authors - Joe Myung Kuk MK
human,mouse	IHC	Cells; Paraoxonase 2 (PON2) Deficiency Reproduces Lipid Alterations of Diabetic and Inflammatory Glomerular Disease and Affects TRPC6 Signaling; Authors - Henning Hagmann
mouse	WB,IF	Biomed Pharmacother; GYY4137, a H2S donor, ameliorates kidney injuries in diabetic mice by modifying renal ROS-associated enzymes; Authors - Xueqi Chen