Anticorps Recombinant de lapin anti-PON2

PON2 Recombinant Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

242673H1

N° de cat : 85126-4-RR

Synonymes

242673H1, A-esterase 2, Aromatic esterase 2, EC:3.1.1.81, PON 2

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Galerie de données de validation

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  WB analysis using 85126-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 85126-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85126-4-RR (Pon2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules L-929, cellules RAW 264.7, tissu cardiaque de rat, tissu pulmonaire de rat, tissu pulmonaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85126-4-RR cible PON2 dans les applications de WB, ELISA et montre une réactivité avec des échantillons rat, souris

• Réactivité: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PON2 Protéine recombinante Eg2741
• Nom complet: paraoxonase 2
• Masse moléculaire calculée: 40 kDa
• Poids moléculaire observé: 39 kDa
• Numéro d’acquisition GenBank: NM_183308.2
• Symbole du gène: Pon2
• Identification du gène (NCBI): 330260
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PON2 (Serum paraoxonase/arylesterase 2) has antioxidant activity and can prevents LDL lipid peroxidation, reverses the oxidation of mildly oxidized LDL, and inhibits the ability of MM-LDL to induce monocyte chemotaxis. Highest levels of PON2 protein are found in the mouse lung and small intestine, followed by the heart and liver, while lower levels are present in the testis, kidney and brain. PON2 expression in tissues from female mice is always significantly higher than in male animals. There are also some reports showing two bands of 43 kDa and 53 kDa to be detected through western blot as the two isoforms of this protein. (PMID:21354197).

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