Anticorps Polyclonal de lapin anti-POP1

• Phare
• Validé par KD/KO

POP1 Polyclonal Antibody for WB, IHC, IF/ICC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 12029-1-PBS

Synonymes

POP 1

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Galerie de données de validation

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  WB analysis using 12029-1-AP (same clone as 12029-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 12029-1-AP (POP1 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

  WB result of POP1 antibody (12029-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-POP1 transfected HeLa cells. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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  IP experiment of HeLa using 12029-1-AP (same clone as 12029-1-PBS)

  IP result of anti-POP1 (IP:12029-1-AP, 4ug; Detection:12029-1-AP 1:8000) with HeLa cells lysate 1560 ug. This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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  IHC staining of human ovary cancer using 12029-1-AP (same clone as 12029-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12029-1-AP (POP1 antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 12029-1-AP (same clone as 12029-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using POP1 antibody (12029-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 12029-1-PBS in a different storage buffer formulation.

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Informations sur le produit

12029-1-PBS cible POP1 dans les applications de WB, IHC, IF/ICC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POP1 Protéine recombinante Ag2660
• Nom complet: processing of precursor 1, ribonuclease P/MRP subunit (S. cerevisiae)
• Masse moléculaire calculée: 1024 aa, 115 kDa
• Poids moléculaire observé: 115 kDa
• Numéro d’acquisition GenBank: BC011529
• Symbole du gène: POP1
• Identification du gène (NCBI): 10940
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Processing of Precursor 1 (POP1), a core component of RNase subunits, is an autoantigen in patients with connective tissue disease and is involved in suppressing inflammation.

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