Anticorps Polyclonal de lapin anti-PORCN

PORCN Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 20613-1-PBS

Synonymes

DHOF, EC:2.3.1.250, FODH, MG61, por

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Galerie de données de validation

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  WB analysis using 20613-1-AP (same clone as 20613-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 20613-1-AP (PORCN antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 20613-1-PBS in a different storage buffer formulation.

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  IHC staining of rat kidney using 20613-1-AP (same clone as 20613-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat kidney tissue slide using 20613-1-AP (PORCN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20613-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 20613-1-AP (same clone as 20613-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 20613-1-AP (PORCN antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 20613-1-PBS in a different storage buffer formulation.

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Informations sur le produit

20613-1-PBS cible PORCN dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PORCN Protéine recombinante Ag14664
• Nom complet: porcupine homolog (Drosophila)
• Masse moléculaire calculée: 461 aa, 52 kDa
• Poids moléculaire observé: 43~50 kDa
• Numéro d’acquisition GenBank: BC019080
• Symbole du gène: PORCN
• Identification du gène (NCBI): 64840
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PORCN is one of several related membrane-bound O-acyltransferase (MBOAT) enzymes encoding a 461 amino acid-sized (52 kDa) protein named porcupine located in the endoplasmic reticulum and known to be involved in the processing, secretion, and signaling of Wnt proteins (PMID: 17546031). Overexpression of PORCN could promote the proliferation and migration abilities of HCC cells both in vitro and in vivo (PMID: 37588740, 23188502).

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