Anticorps Polyclonal de lapin anti-POU2AF1

POU2AF1 Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 13605-1-AP

Synonymes

B-cell-specific coactivator OBF-1, BOB1, BOB-1, OBF1, OCAB

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Galerie de données de validation

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  WB analysis of mouse lung using 13605-1-AP

  mouse lung tissue was subjected to SDS PAGE followed by western blot with 13605-1-AP (POU2AF1 antibody) at dilution of 1:500 and incubated at room temperature for 1.5 hours.

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  WB analysis using 13605-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 13605-1-AP (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours.

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  IF Staining of Raji using 13605-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Raji cells using POU2AF1 antibody (13605-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2).

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Applications testées

• Résultats positifs en WB: cellules Raji, tissu pulmonaire de souris
• Résultats positifs en IF/ICC: cellules Raji,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

13605-1-AP cible POU2AF1 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POU2AF1 Protéine recombinante Ag4482
• Nom complet: POU class 2 associating factor 1
• Masse moléculaire calculée: 256 aa, 27 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC032549
• Symbole du gène: POU2AF1
• Identification du gène (NCBI): 5450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

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