Anticorps Monoclonal anti-POU2AF1

POU2AF1 Monoclonal Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

3C5A7

N° de cat : 66659-1-PBS

Synonymes

3C5A7, B-cell-specific coactivator OBF-1, BOB1, BOB-1, OBF1

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Galerie de données de validation

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  WB analysis of Raji using 66659-1-Ig (same clone as 66659-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 66659-1-Ig (POU2AF1 antibody) at dilution of 1:5000 and incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66659-1-PBS in a different storage buffer formulation.

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  IHC staining of Human tonsillitis using 66659-1-Ig (same clone as 66659-1-PBS)

  Immunohistochemical analysis of paraffin-embedded Human tonsillitis tissue slide using 66659-1-Ig (POU2AF1 antibody) at dilution of 1:1700 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 66659-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51058-1

  Cytometric bead array standard curve of MP51058-1, POU2AF1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66659-2-PBS. Detection antibody: 66659-1-PBS. Standard:Ag23613. Range: 0.781-200 ng/mL.

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  Cytometric bead array standard curve of MP51058-2

  Cytometric bead array standard curve of MP51058-2, POU2AF1 Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 66659-1-PBS. Detection antibody: 66659-3-PBS. Standard:Ag23613. Range: 0.781-200 ng/mL.

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Informations sur le produit

66659-1-PBS cible POU2AF1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: POU2AF1 Protéine recombinante Ag23613
• Nom complet: POU class 2 associating factor 1
• Masse moléculaire calculée: 256 aa, 27 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC032549
• Symbole du gène: POU2AF1
• Identification du gène (NCBI): 5450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

POU2AF1, also named as BOB-1 or OCA-B, is a 256 amino acid protein, which belongs to the POU2AF1 family. POU2AF1 is expressed in B-cell specific. POU2AF1 as a transcriptional coactivator that specifically associates with either OCT1 or OCT2. It boosts the OCT1 mediated promoter activity and to a lesser extent, that of OCT2. It has no intrinsic DNA-binding activity. It recognizes the POU domains of OCT1 and OCT2. It is essential for the response of B-cells to antigens and required for the formation of germinal centers.The predicted size of this protein is 27 kDa. Studies have reported that the protein has a multi-band size of 34-35 kDa through siRNA interference experiments (PMID: 17621271). This result is the same as our experiments.

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