Anticorps Polyclonal de lapin anti-OCT1

• Phare
• Validé par KD/KO

OCT1 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (3)

Applications

WB, IP, IHC, IF, ChIP, ELISA

Conjugaison

Non conjugué

Publications

13

N° de cat : 10387-1-AP

Synonymes

NF A1, Oct 1, OCT1, Octamer binding protein 1, OTF 1, OTF1, POU class 2 homeobox 1, POU2F1

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Galerie de données de validation

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  WB analysis using 10387-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10387-1-AP (OCT1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

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  WB analysis of NCCIT using 10387-1-AP

  NCCIT cell were subjected to SDS PAGE followed by western blot with 10387-1-AP (OCT1 antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of HeLa using 10387-1-AP

  IP Result of anti-OCT1 (IP:10387-1-AP, 4ug; Detection:10387-1-AP 1:300) with HeLa cells lysate 1600ug.

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  IHC staining of human colon cancer using 10387-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10387-1-AP (OCT1 antibody at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lymphoma using 10387-1-AP

  Immunohistochemical analysis of paraffin-embedded human lymphoma using 10387-1-AP (OCT1 antibody) at dilution of 1:100 (under 25x lens).

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  IHC staining of human colon cancer using 10387-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 10387-1-AP (OCT1 antibody at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HeLa using 10387-1-AP

  Immunofluorescent analysis of HeLa cells using 10387-1-AP (OCT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 10387-1-AP

  Immunofluorescent analysis of HeLa cells using 10387-1-AP (OCT1 antibody) at dilution of 1:25 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa, cellules HepG2, cellules NCCIT
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de lymphome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 11 publications below
• IF: See 1 publications below
• ChIP: See 2 publications below

Informations sur le produit

10387-1-AP cible OCT1 dans les applications de WB, IP, IHC, IF, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: OCT1 Protéine recombinante Ag0586
• Nom complet: POU class 2 homeobox 1
• Masse moléculaire calculée: 76 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC003571
• Symbole du gène: POU2F1
• Identification du gène (NCBI): 5451
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

The POU class 2 homeobox 1 (POU2F1), also known as octamer-binding transcription factor-1 (OCT-1), is a ubiquitous nuclear protein that regulates the transcription of target genes by binding to a cis-acting octamer element(5'-ATTTGCAT-3'). It participated in the regulation of housekeeping genes, including histone H2B and snRNAs, for cell differentiation and proliferation [PMID:3462701]. In addition, it functions as a cellular stress sensor that modulates activities of tissue-specific target genes involved in immune and inflammatory responses [PMID:16322220]. It also acts as a repressor for the cytokine-inducible, NF-kB-dependent expression of E-selectin and the vascular cell adhesion molecule during vascular inflammation. Otherwise, it can interact with nuclear hormone receptors, such as the retinoid X receptor, the thyroid hormone receptor and the glucocorticoid receptor, and influence their transcription activity[PMID:17192276].

Publications

Species	Application	Title
pig	ChIP	Mol Biol Evol; Long-term artificial selection reveals a role of TCTP in autophagy in mammalian cells.; Authors - Ke Chen
mouse	IF	Food Funct; Anti-hyperuricemic potential of stevia (Stevia rebaudiana Bertoni) residue extract in hyperuricemic mice; Authors - Arshad Mehmood
rat	WB	Food Funct; Ferulic acid supplementation alleviates hyperuricemia in high-fructose/fat diet-fed rats via promoting uric acid excretion and mediating the gut microbiota; Authors - Nanhai Zhang
human	WB	Cell Oncol (Dordr); The POU2F1/miR-4490/USP22 axis regulates cell proliferation and metastasis in gastric cancer.; Authors - Yizhi Xiao
human	WB	iScience; MASTL is enriched in cancerous and pluripotent stem cells and influences OCT1/OCT4 levels.; Authors - Elisa Närvä ; KD Validated
mouse	WB	Mol Cell Endocrinol; MiR-451a attenuates free fatty acids-mediated hepatocyte steatosis by targeting the thyroid hormone responsive spot 14 gene.; Authors - Ni Zeng