Anticorps Polyclonal de lapin anti-BRN2

BRN2 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

5

Avis (1)

N° de cat : 14596-1-AP

Synonymes

Brain 2, Brn 2, BRN2, Oct 7, OCT7, Octamer binding protein 7, OTF 7, OTF7, POU class 3 homeobox 2, POU3F2, POUF3

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Galerie de données de validation

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  WB analysis of HeLa using 14596-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14596-1-AP (BRN2 antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  WB analysis of Jurkat using 14596-1-AP

  Jurkat cells were subjected to SDS PAGE followed by western blot with 14596-1-AP (BRN2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human gliomas using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human gliomas using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human gliomas tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human spleen using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human ovary using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human ovary using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of mouse brain using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14596-1-AP (BRN2 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human placenta using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human skin using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 40x lens).

• 

  IHC staining of human spleen using 14596-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14596-1-AP (BRN2 antibody) at dilution of 1:100 (under 10x lens).

• 

  IF Staining of mouse brain using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of mouse brain using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human gliomas using 14596-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human gliomas tissue using BRN2 antibody (14596-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules Jurkat
• Résultats positifs en IHC: tissu cérébral de souris, tissu cutané humain, tissu de gliome humain, tissu ovarien humain, tissu placentaire humain, tissu splénique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris, tissu de gliome humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

14596-1-AP cible BRN2 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: BRN2 Protéine recombinante Ag6131
• Nom complet: POU class 3 homeobox 2
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 47 kDa
• Numéro d’acquisition GenBank: BC051699
• Symbole du gène: POU3F2
• Identification du gène (NCBI): 5454
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POU3F2 belongs to a large family of transcription factors that bind to the octameric DNA sequence ATGCAAAT. It shares a highly homologous region that referred to as the POU domain. POU3F2 is a Class III POU genes that expressed predominantly in the central nervous system (CNS). It is likely that CNS-specific transcription factors such as these play an important role in mammalian neurogenesis by regulating their diverse patterns of gene expression. POU3F2 is required for maintaining neural cell differetiation and has a role in the production and positioning of neocortical neurons.

Publications

Species	Application	Title
mouse	IF	Hum Mol Genet; Glutathione S-transferase Pi (Gstp) proteins regulate neuritogenesis in the developing cerebral cortex.; Authors - Xiaonan Liu
rat	WB	FEBS Lett; miR-221/222 suppression induced by activation of the cAMP/PKA/CREB1 pathway is required for cAMP-induced bidirectional differentiation of glioma cells.; Authors - Qian Liu
mouse	IF	Neurosci Lett; Overexpression of the 14-3-3gamma protein in embryonic mice results in neuronal migration delay in the developing cerebral cortex.; Authors - Brett Cornell
mouse	IF	Neuron; HDAC1 and HDAC2 Regulate Intermediate Progenitor Positioning to Safeguard Neocortical Development.; Authors - Tianxiang Tang
mouse	IF	Adv Sci (Weinh); Enabling Survival of Transplanted Neural Precursor Cells in the Ischemic Brain; Authors - Zhifu Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Mandi (Verified Customer) (09-26-2019)

Positive staining in human cortical neurons derived from iPSCs

• Applications: Immunofluorescence,
• Primary Antibody Dilution: 1:500