Anticorps Polyclonal de lapin anti-POU4F2

POU4F2 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(4)

Avis (1)

N° de cat : 55042-1-AP

Synonymes

Brn-3B, BRN3B, Brain-specific homeobox/POU domain protein 3B, Brain-3B

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Galerie de données de validation

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  WB analysis of mouse brain using 55042-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 55042-1-AP (POU4F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 55042-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 55042-1-AP (POU4F2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse eye using 55042-1-AP

  mouse eye tissue were subjected to SDS PAGE followed by western blot with 55042-1-AP (POU4F2 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules L02, tissu cérébral de souris, tissu oculaire de souris

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 2 publications below

Informations sur le produit

55042-1-AP cible POU4F2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: POU class 4 homeobox 2
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 40-44 kDa
• Numéro d’acquisition GenBank: NM_004575
• Symbole du gène: POU4F2
• Identification du gène (NCBI): 5458
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

POU4F2,also named as BRN3.2, BRN3B and Brn-3b, belongs to the POU transcription factor family and Class-4 subfamily. It is a transcription factor. POU4F2 may play a role in determining or maintaining the identities of a small subset of visual system neurons. The antibody is specific to POU4F2.

Publications

Species	Application	Title
		Stem Cell Res Ther; Protective effects of human iPS-derived retinal pigmented epithelial cells on retinal degenerative disease.; Authors - Deliang Zhu
mouse	WB,IF	Sci Rep; 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.; Authors - Zuberwasim Sayyad
human	WB, IF	Neurochem Int; Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma.; Authors - Zuberwasim Sayyad
human	WB	Life Sci; Intervention of epithelial mesenchymal transition against colon cancer cell growth and metastasis based on SOX21/POU4F2/Hedgehog signaling axis; Authors - Qiaochang Cao

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Alessandro (Verified Customer) (12-09-2023)

good Ab for retinal ganglio cells

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:300
• Cell Tissue Type: Retinal organoids

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