Anticorps Polyclonal de lapin anti-POU4F3

POU4F3 Polyclonal Antibody for WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

6

Avis (1)

N° de cat : 21509-1-AP

Synonymes

Brain 3C, Brn 3C, BRN3C, DFNA15, POU class 4 homeobox 3, POU4F3

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Galerie de données de validation

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  WB analysis of Neuro-2a using 21509-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HL-60 using 21509-1-AP

  HL-60 cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of Neuro-2a using 21509-1-AP

  Neuro-2a cells were subjected to SDS PAGE followed by western blot with 21509-1-AP (POU4F3 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Neuro-2a, cellules HL-60

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 4 publications below

Informations sur le produit

21509-1-AP cible POU4F3 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: POU4F3 Protéine recombinante Ag16004
• Nom complet: POU class 4 homeobox 3
• Masse moléculaire calculée: 338 aa, 37 kDa
• Poids moléculaire observé: 35-40 kDa
• Numéro d’acquisition GenBank: BC104923
• Symbole du gène: POU4F3
• Identification du gène (NCBI): 5459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Cell; Glia-to-Neuron Conversion by CRISPR-CasRx Alleviates Symptoms of Neurological Disease in Mice.; Authors - Haibo Zhou
mouse	IHC	Front Cell Dev Biol; In vivo Regeneration of Ganglion Cells for Vision Restoration in Mammalian Retinas.; Authors - Dongchang Xiao
mouse	WB,IF	Sci Rep; 661W is a retinal ganglion precursor-like cell line in which glaucoma-associated optineurin mutants induce cell death selectively.; Authors - Zuberwasim Sayyad
human	WB, IF	Neurochem Int; Human primary retinal cells as an in-vitro model for investigating defective signalling caused by OPTN mutants associated with glaucoma.; Authors - Zuberwasim Sayyad
mouse	IF	Hear Res; Sox2 is required in supporting cells for normal levels of vestibular hair cell regeneration in adult mice; Authors - Amanda N Ciani Berlingeri
mouse		Elife; Cellular reprogramming with ATOH1, GFI1, and POU4F3 implicate epigenetic changes and cell-cell signaling as obstacles to hair cell regeneration in mature mammals; Authors - Amrita A Iyer

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

AMRITA (Verified Customer) (02-15-2021)

The antibody requires a prior antigen retrieval step for 15min in 80 deg centigrade

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: Inner ear - organ of Corti hair cells stained

POU4F3 Antibody Immunohistochemistry validation (1:200 dilution) in Inner ear - organ of Corti hair cells stained (Cat no:21509-1-AP)