Anticorps Recombinant de lapin anti-POU4F3

POU4F3 Recombinant Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

230345F1

N° de cat : 82980-1-PBS

Synonymes

230345F1, Brain 3C, Brain-3C, Brain-specific homeobox/POU domain protein 3C, Brn 3C

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Galerie de données de validation

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  WB analysis using 82980-1-RR (same clone as 82980-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 82980-1-RR (POU4F3 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 82980-1-RR (same clone as 82980-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 82980-1-RR (POU4F3 antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IF Staining of rat brain using 82980-1-RR (same clone as 82980-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed paraffin-embedded rat brain tissue using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:350 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 82980-1-RR (same clone as 82980-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using POU4F3 antibody (82980-1-RR, Clone: 230345F1 ) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  FC experiment of U-251 using 82980-1-RR (same clone as 82980-1-PBS)

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug Anti-Human POU4F3 (82980-1-RR, Clone:230345F1) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed and permeabilized with True-Nuclear Transcription Factor Buffer Set. This data was developed using the same antibody clone with 82980-1-PBS in a different storage buffer formulation.

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  Affinity and Kinetic Characterization of 82980-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82980-1-RR against Human POU4F3 were performed. The affinity constant is 0.234 nM.

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Informations sur le produit

82980-1-PBS cible POU4F3 dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: POU4F3 Protéine recombinante Ag16004
• Nom complet: POU class 4 homeobox 3
• Masse moléculaire calculée: 338 aa, 37 kDa
• Poids moléculaire observé: 33-40 kDa
• Numéro d’acquisition GenBank: BC104923
• Symbole du gène: POU4F3
• Identification du gène (NCBI): 5459
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

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