Anticorps Monoclonal anti-PPA1

PPA1 Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

1D11F4

N° de cat : 68178-1-PBS

Synonymes

Inorganic pyrophosphatase, IOPPP, PP, PP1, PPA1, PPase, pyrophosphatase (inorganic) 1, SID6 8061

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Galerie de données de validation

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  WB analysis using 68178-1-Ig (same clone as 68178-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68178-1-Ig (PPA1 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 68178-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68178-1-Ig (same clone as 68178-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed HeLa cells using PPA1 antibody (68178-1-Ig, Clone: 1D11F4 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68178-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68178-1-PBS cible PPA1 dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPA1 Protéine recombinante Ag7049
• Nom complet: pyrophosphatase (inorganic) 1
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33 kDa
• Numéro d’acquisition GenBank: BC001022
• Symbole du gène: PPA1
• Identification du gène (NCBI): 5464
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PPA1(Inorganic pyrophosphatase) is also named as IOPPP, PP and PPase. It maintains the thermodynamic favorability of these reactions by catalyzing the hydrolysis of pyrophosphates into organic phosphates, which are then exported across the cell membrane(PMID:16300924).The erythrocyte inorganic pyrophosphatase molecule would appear to be a dimer(PMID:4130389).

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