Anticorps Polyclonal de lapin anti-PPA2

• Phare
• Validé par KD/KO

PPA2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(1)

N° de cat : 16662-1-AP

Synonymes

EC:3.6.1.1, HSPC124, PPase 2, Pyrophosphatase SID6 306, Pyrophosphatase SID6-306

---

Galerie de données de validation

• 

  WB analysis of HEK-293 using 16662-1-AP

  WB result of PPA2 antibody (16662-1-AP; 1:4000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPA2 transfected HEK-293 cells.

• 

  WB analysis of mouse heart using 16662-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16662-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 16662-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 16662-1-AP (PPA2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 16662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 16662-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16662-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 16662-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 16662-1-AP (PPA2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

---

Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HEK-293, tissu cardiaque de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu de cancer du poumon humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 1 publications below

Informations sur le produit

16662-1-AP cible PPA2 dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPA2 Protéine recombinante Ag10138
• Nom complet: pyrophosphatase (inorganic) 2
• Masse moléculaire calculée: 305aa,35 kDa; 334aa,38 kDa
• Poids moléculaire observé: 32-40 kDa
• Numéro d’acquisition GenBank: BC057219
• Symbole du gène: PPA2
• Identification du gène (NCBI): 27068
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Pyrophosphatase 2 (PPA2) is a mitochondrial-located pyrophosphatase (PPase) that hydrolyzes inorganic pyrophosphate (PPi), generated by numerous nucleotide-dependent reactions, into two orthophosphate molecules (PMID: 34400813). PPA2 is a cardiomyopathy-associated protein, which has a greater physiological importance in mitochondrial function (PMID: 27523597).

Publications

Species	Application	Title
human	IHC	Front Immunol; Multi-omics pan-cancer analysis reveals the prognostic values and immunological functions of PPA2, with a spotlight on breast cancer; Authors - Jia-Ning Zhang

{{ptg:RelatedPrimaryAntibodies}}