Anticorps Monoclonal anti-PPARA

• Phare
• Validé par KD/KO

PPARA Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

1G1E10

N° de cat : 66826-1-PBS

Synonymes

PPAR alpha, PPAR, Peroxisome proliferator-activated receptor alpha, NR1C1, hPPAR

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Galerie de données de validation

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  WB analysis of ROS1728 using 66826-1-Ig (same clone as 66826-1-PBS)

  ROS1728 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66826-1-PBS in a different storage buffer formulation.

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  WB analysis of HSC-T6 using 66826-1-Ig (same clone as 66826-1-PBS)

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66826-1-Ig (PPARA antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 66826-1-PBS in a different storage buffer formulation.

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Informations sur le produit

66826-1-PBS cible PPARA dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPARA Protéine recombinante Ag7896
• Nom complet: peroxisome proliferator-activated receptor alpha
• Masse moléculaire calculée: 52 kDa
• Poids moléculaire observé: 53 kDa
• Numéro d’acquisition GenBank: BC000052
• Symbole du gène: PPARA
• Identification du gène (NCBI): 5465
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome proliferator-activated receptor alpha (PPARA) is a ligand-activated transcription factor that belongs to the PPAR nuclear receptor superfamily. PPARA is essential in the modulation of lipid transport and metabolism, mainly through activating mitochondrial and peroxisomal fatty acid β-oxidation pathways. In addition, PPARA seems to decrease inflammation mainly through direct interaction with NF-κB, causing inhibition of its signaling pathway or reducing the activated levels of NF-κB and subsequent inflammation. Furthermore, PPARA was implicated in the attenuation of oxidative stress in alcoholic liver disease when treated with polyenephosphatidylcholine through downregulation of ROS-generating enzymes such as ethanol-inducible cytochrome P450 2E1 (CYP2E1), acyl-CoA oxidase, and NADPH oxidase. PPARA exists two isoforms and molecular weight of PPARA isoforms are 52 kDa and 22 kDa. The ability of a retinoid X receptor (RXR) to heterodimerize with many nuclear receptors, including LXR, PPAR, NGF1B and RAR, underscores its pivotal role within the nuclear receptor superfamily. Among these heterodimers, PPAR:RXR is considered an important signalling mediator of both PPAR ligands, such as fatty acids, and 9-cis retinoic acid (9-cis RA), an RXR ligand. (PMID: 15103326 ). PPARA can form Heterodimer with RXRA and molecular weight of Heterodimer is about 110 kDa.

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