Anticorps Monoclonal anti-PPARD

• Phare
• Validé par KD/KO

PPARD Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

6

CloneNo.

1B10E1

N° de cat : 60193-1-Ig

Synonymes

FAAR, NR1C2, NUC1, NUCI, NUCII, Nuclear hormone receptor 1, PPAR beta, PPAR delta, PPARB, PPARD

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Galerie de données de validation

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  WB analysis using 60193-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of A549 using 60193-1-Ig

  WB result of PPARD antibody (60193-1-Ig; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPARD transfected A549 cells.

• 

  WB analysis using 60193-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat testis using 60193-1-Ig

  rat testis tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of SW480 using 60193-1-Ig

  SW480 cells were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of pig adipose using 60193-1-Ig

  pig adipose tissue were subjected to SDS PAGE followed by western blot with 60193-1-Ig (PPARD antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules A549, cellules Jurkat, cellules K-562, tissu adipeux de porc

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below

Informations sur le produit

60193-1-Ig cible PPARD dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPARD Protéine recombinante Ag16770
• Nom complet: peroxisome proliferator-activated receptor delta
• Masse moléculaire calculée: 50 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC002715
• Symbole du gène: PPARD
• Identification du gène (NCBI): 5467
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome proliferator-activator receptor delta(PPARD), a ligand-activated transcription factor, belongs to the PPARs family, which get its name for their chemicals that induce proliferation of peroxisomes, organelles that contributes to the oxidation of fatty acids. PPARD is a receptor for peroxisome proliferator and once activated by a ligand, it binds to and activates the promoter elements of target genes, such as acylCoA oxidase gene. But it act as a repressor for the NPC1L1 gene.

Publications

Species	Application	Title
mouse	WB	Nat Commun; A proteomics landscape of circadian clock in mouse liver.; Authors - Yunzhi Wang
mouse	WB	Liver Int; PPARβ/δ activation protects against hepatic ischaemia-reperfusion injury; Authors - Baolin Qian ; KD Validated
mouse	WB	Am J Physiol Endocrinol Metab; Zinc finger protein 407 overexpression upregulates PPAR target gene expression and improves glucose homeostasis in mice; Authors - Alyssa Charrier
mouse	WB	Front Aging Neurosci; A tau fragment links depressive-like behaviors and cognitive declines in Alzheimer's disease mouse models through attenuating mitochondrial function; Authors - Yamei Wang
human	WB	Clin Transl Med; Cullin-associated and neddylation-dissociated 1 regulate reprogramming of lipid metabolism through SKP1-Cullin-1-F-boxFBXO11 -mediated heterogeneous nuclear ribonucleoprotein A2/B1 ubiquitination and promote hepatocellular carcinoma; Authors - Hao Zhang
mouse	WB	iScience; WTAP boosts lipid oxidation and induces diabetic cardiac fibrosis by enhancing AR methylation; Authors - Kai Song