Anticorps Polyclonal de lapin anti-PPAR Gamma

• Phare
• Validé par KD/KO

PPAR Gamma Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

9

N° de cat : 22061-1-AP

Synonymes

CIMT1, NR1C3, PPAR gamma, PPARG, PPARG2

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Galerie de données de validation

• 

  WB analysis using 22061-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR Gamma antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 22061-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 22061-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of 3T3-L1 using 22061-1-AP

  3T3-L1 cells were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human brain using 22061-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 22061-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human heart using 22061-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse ovary using 22061-1-AP

  mouse ovary tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 22061-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 22061-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 22061-1-AP (PPAR gamma antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human prostate cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human colon cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22061-1-AP (PPAR gamma antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of rat colon using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat colon using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded rat colon tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 22061-1-AP (PPAR Gamma antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human placenta using 22061-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 22061-1-AP (PPAR gamma antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules 3T3-L1, cellules HL-60, cellules MCF-7, tissu cardiaque de souris, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu cérébral humain, tissu hépatique de rat, tissu hépatique de souris, tissu ovarien de souris, tissu testiculaire de souris
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu de cancer du côlon humain, tissu de côlon de rat, tissu placentaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below
• IHC: See 1 publications below

Informations sur le produit

22061-1-AP cible PPAR Gamma dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPAR Gamma Protéine recombinante Ag17136
• Nom complet: peroxisome proliferator-activated receptor gamma
• Masse moléculaire calculée: 505 aa, 58 kDa
• Poids moléculaire observé: 66-70 kDa
• Numéro d’acquisition GenBank: BC006811
• Symbole du gène: PPARG
• Identification du gène (NCBI): 5468
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Peroxisome Proliferator-Activated Receptors (PPARs) are ligand-activated intracellular transcription factors, members of the nuclear hormone receptor superfamily (NR), that includes estrogen, thyroid hormone receptors, retinoic acid, Vitamin D3 as well as retinoid X receptors (RXRs). The PPAR subfamily consists of three subtypes encoded by distinct genes denoted PPARα (NR1C1), PPARβ/δ (NR1C2) and PPARγ (NR1C3), which are activated by selective ligands. PPARγ, also named as PPARG, contains one nuclear receptor DNA-binding domain and is a receptor that binds peroxisome proliferators such as hypolipidemic drugs and fatty acids. It plays an important role in the regulation of lipid homeostasis, adipogenesis, INS resistance, and development of various organs. Defects in PPARG are the cause of familial partial lipodystrophy type 3 (FPLD3) and may be associated with susceptibility to obesity. Defects in PPARG can lead to type 2 INS-resistant diabetes and hypertension. PPARG mutations may be associated with colon cancer. Genetic variations in PPARG are associated with susceptibility to glioma type 1 (GLM1). PPARG has two isoforms with molecular weight 57 kDa and 54 kDa (PMID: 9831621), but modified PPARG is about 67 KDa (PMID: 16809887). PPARG2 is a splice variant and has an additional 30 amino acids at the N-terminus (PMID: 15689403). Experimental data indicate that a 45 kDa protein displaying three different sequences immunologically related to the nuclear receptor PPARG2 is located in mitochondria (mt-PPAR). However, the molecular weight of this protein is clearly less when compared to that of PPARG2 (57 kDa). (PMID: 10922459). PPARG has been reported to be localized mainly (but not always) in the nucleus. PPARG can also be detected in the cytoplasm and was reported to possess extra-nuclear/non-genomic actions (PMID: 17611413; 19432669; 14681322).

Publications

Species	Application	Title
mouse	WB	Autophagy; Mir223 restrains autophagy and promotes CNS inflammation by targeting ATG16L1.; Authors - Yan Li
mouse	WB	Genes Dev; Loss of TLE3 promotes the mitochondrial program in beige adipocytes and improves glucose metabolism.; Authors - Stephanie Pearson
mouse	WB	Free Radic Biol Med; Hydrogen sulfide alleviates particulate matter-induced emphysema and airway inflammation by suppressing ferroptosis.; Authors - Ying Wang
mouse	WB	Biochem Pharmacol; A novel pyrazole-containing indolizine derivative suppresses NF-κB activation and protects against TNBS-induced colitis via a PPAR-γ-dependent pathway.; Authors - Yong Fu
		Front Pharmacol; The Active Components of Fuzheng Huayu Formula and Their Potential Mechanism of Action in Inhibiting the Hepatic Stellate Cells Viability - A Network Pharmacology and Transcriptomics Approach.; Authors - Xinrui Xing
rat	WB	Front Pharmacol; Alpha-Asaronol Alleviates Dysmyelination by Enhancing Glutamate Transport Through the Activation of PPARγ-GLT-1 Signaling in Hypoxia-Ischemia Neonatal Rats.; Authors - Yuhang Ge

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