Anticorps Monoclonal anti-PGC1a

• Phare
• Validé par KD/KO

PGC1a Monoclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (5)

Applications

WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA

Conjugaison

Non conjugué

Publications(528)

CloneNo.

1C1B2

Avis (5)

N° de cat : 66369-1-Ig

Synonymes

PPARGC1A, 1C1B2, LEM6, Ligand effect modulator 6, PGC 1 alpha

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Galerie de données de validation

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  WB analysis using 66369-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66369-1-Ig

  WB result of PGC1a antibody (66369-1-Ig; 1:8000; room temperature for 1.5 hours) with wild-type and PGC1a knockout HeLa cells.

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  WB analysis of HSC-T6 using 66369-1-Ig

  HSC-T6 cells were subjected to SDS PAGE followed by western blot with 66369-1-Ig (PGC1a antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 66369-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PGC1a antibody (66369-1-Ig, Clone: 1C1B2 ) at dilution of 1:2000 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Mouse Recombinant Secondary Antibody (H+L) (RGAM002).

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Applications testées

• Résultats positifs en WB: tissu de muscle squelettique de porc, cellules HSC-T6, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 15 publications below
• WB: See 486 publications below
• IHC: See 33 publications below
• IF: See 69 publications below
• IP: See 8 publications below
• ELISA: See 1 publications below
• CoIP: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

66369-1-Ig cible PGC1a dans les applications de WB, IHC, IF/ICC, IP, CoIP, ChIP, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, porc, poulet, singe, souris, Hamster
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
• Masse moléculaire calculée: 91 kDa
• Poids moléculaire observé: 70 kDa, 100 kDa
• Numéro d’acquisition GenBank: NM_013261
• Symbole du gène: PGC1a
• Identification du gène (NCBI): 10891
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

Publications

Species	Application	Title
mouse	WB	Nature; A plant-derived natural photosynthetic system for improving cell anabolism; Authors - Pengfei Chen
mouse	WB	Nat Aging; Activation of AMPK by GLP-1R agonists mitigates Alzheimer-related phenotypes in transgenic mice; Authors - Yun Zhang
mouse	WB	Nat Commun; Moderate-intensity interval exercise exacerbates cardiac lipotoxicity in high-fat, high-calories diet-fed mice; Authors - Jing Geng
human	ChIP	Adv Sci (Weinh); Sirtuin1 Suppresses Calcium Oxalate Nephropathy via Inhibition of Renal Proximal Tubular Cell Ferroptosis Through PGC-1α-mediated Transcriptional Coactivation; Authors - Chen Duan
mouse	WB	Sci Adv; Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.; Authors - Jun Seok Son
chicken	WB,IF	J Hazard Mater; Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers; Authors - Gaolong Zhong

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Michaela (Verified Customer) (11-24-2023)

Worked well in 15μg mouse brain tissue lysate in Western Blot. Band appears between 60-100 kDa

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: Mouse brain tissue

FH

No (Verified Customer) (10-24-2023)

A good antibody to detect endogenous PGC1a.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: mouse brain lysate

PGC1a Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66369-1-Ig)

FH

Mi (Verified Customer) (03-07-2023)

It doesn´t work well in human white and brown adipocyte cells, we got bands at unexpected size. Further KO validation may be needed.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: adipocytes

FH

Huai-Chin (Verified Customer) (07-11-2022)

There are several non-specific bands. At least there's a dominant band after adipogenesis and we can use this antibody at a fairly high dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:10,000
• Cell Tissue Type: 3T3-L1 cells

FH

Mohammad (Verified Customer) (01-21-2020)

It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.

• Applications: Western Blot,
• Cell Tissue Type: Mouse hippocampus

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