• Phare
  • Validé par KD/KO

Anticorps Monoclonal anti-PGC1a

PGC1a Monoclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (4)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

CloneNo.

1C1B2

N° de cat : 66369-1-Ig

Synonymes

LEM6, Ligand effect modulator 6, PGC 1 alpha, PGC 1(alpha), PGC 1v, PGC1, PGC1A, PGC-1α, PPAR gamma coactivator 1 alpha, PPARGC 1 alpha, PPARGC1, PPARGC1A



Applications testées

Résultats positifs en WBtissu de muscle squelettique de porc, cellules C2C12, cellules HSC-T6, cellules ROS1728, tissu de muscle squelettique de rat, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
Résultats positifs en IFcellules HeLa

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunofluorescence (IF)IF : 1:50-1:500
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66369-1-Ig cible PGC1a dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéerat, bovin, Chèvre, Humain, porc, poulet, souris, Hamster
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Peptide
Nom complet peroxisome proliferator-activated receptor gamma, coactivator 1 alpha
Masse moléculaire calculée 91 kDa
Poids moléculaire observé 70 kDa, 100 kDa
Numéro d’acquisition GenBankNM_013261
Symbole du gène PPARGC1A
Identification du gène (NCBI) 10891
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine A
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPARGC1A, also named as Peroxisome proliferator-activated receptor gamma coactivator 1-alpha, is a 798 amino acid protein, which Contains 1 RRM (RNA recognition motif) domain and localizes in the nucleus. PPARGC1A is transcriptional coactivator for steroid receptors and nuclear receptors. PPARGC1A can regulate key mitochondrial genes that contribute to the program of adaptive thermogenesis and plays an essential role in metabolic reprogramming in response to dietary availability through coordination of the expression of a wide array of genes involved in glucose and fatty acid metabolism. PPARGC1A exists an isoform in the liver and the molecular weight of it is 77 kDa. Except for a deletion of 127 amino acids at the N terminus, the protein, termed L-PGC-1 α, is identical to PGC-1.

Protocole

Product Specific Protocols
WB protocol for PGC1a antibody 66369-1-IgDownload protocol
IF protocol for PGC1a antibody 66369-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Nature

A plant-derived natural photosynthetic system for improving cell anabolism

Authors - Pengfei Chen
mouseWB

Sci Adv

Maternal exercise via exerkine apelin enhances brown adipogenesis and prevents metabolic dysfunction in offspring mice.

Authors - Jun Seok Son
chickenWB,IF

J Hazard Mater

Mitochondrial miR-12294-5p regulated copper-induced mitochondrial oxidative stress and mitochondrial quality control imbalance by targeted inhibition of CISD1 in chicken livers

Authors - Gaolong Zhong
WB

J Autoimmun

Autoantibodies against the NineTeen complex and U5 RNP in systemic sclerosis

Authors - Jean-Baptiste Vulsteke
mouseWB

Nat Commun

Imprinted lncRNA Dio3os preprograms intergenerational brown fat development and obesity resistance.

Authors - Yan-Ting Chen
mouseWB

Nat Commun

METTL3 is essential for postnatal development of brown adipose tissue and energy expenditure in mice.

Authors - Yuqin Wang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Michaela (Verified Customer) (11-24-2023)

Worked well in 15μg mouse brain tissue lysate in Western Blot. Band appears between 60-100 kDa

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: Mouse brain tissue
FH

No (Verified Customer) (10-24-2023)

A good antibody to detect endogenous PGC1a.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:2000
  • Cell Tissue Type: mouse brain lysate
PGC1a Antibody Western Blot validation (1:2000 dilution) in mouse brain lysate (Cat no:66369-1-Ig)
FH

Mi (Verified Customer) (03-07-2023)

It doesn´t work well in human white and brown adipocyte cells, we got bands at unexpected size. Further KO validation may be needed.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: adipocytes
FH

Huai-Chin (Verified Customer) (07-11-2022)

There are several non-specific bands. At least there's a dominant band after adipogenesis and we can use this antibody at a fairly high dilution.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:10,000
  • Cell Tissue Type: 3T3-L1 cells
FH

Mohammad (Verified Customer) (01-21-2020)

It was working with some non-specific bands in lower weight. But after 9 months antibody was degraded in -20.

  • Applications: Western Blot,
  • Cell Tissue Type: Mouse hippocampus