Anticorps Monoclonal anti-PPARGC1B

PPARGC1B Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

2D2E8

N° de cat : 67821-1-Ig

Synonymes

ERRL1, PERC, PGC 1 beta, PGC 1(beta), PGC1, PGC1B, PPAR gamma coactivator 1 beta, PPARGC 1 beta, PPARGC1, PPARGC1B

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Galerie de données de validation

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  WB analysis using 67821-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of SH-SY5Y using 67821-1-Ig

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig brain using 67821-1-Ig

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat brain using 67821-1-Ig

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 67821-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 67821-1-Ig

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 67821-1-Ig

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67821-1-Ig (PPARGC1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules MCF-7, cellules SH-SY5Y, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below

Informations sur le produit

67821-1-Ig cible PPARGC1B dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPARGC1B Protéine recombinante Ag28861
• Nom complet: peroxisome proliferator-activated receptor gamma, coactivator 1 beta
• Masse moléculaire calculée: 1023 aa, 113 kDa
• Poids moléculaire observé: 150 kDa
• Numéro d’acquisition GenBank: BC132971
• Symbole du gène: PPARGC1B
• Identification du gène (NCBI): 133522
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
rat	WB	Chem Biol Interact; N-acetylcysteine delayed cadmium-induced chronic kidney injury by activating the sirtuin 1-P53 signaling pathway; Authors - Wenxuan Dong
mouse	WB	Sci Rep; Dietary oleic acid intake increases the proportion of type 1 and 2X muscle fibers in mice; Authors - Yusuke Komiya