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Anticorps Polyclonal de lapin anti-PPCDC

PPCDC Polyclonal Antibody for WB, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16270-1-PBS

Synonymes

Galerie de données de validation

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  • Western Blot (WB) analysis of human placenta tissue using PPCDC Polyclonal antibody (16270-1-AP)

    WB analysis of human placenta using 16270-1-AP (same clone as 16270-1-PBS)

    human placenta tissue were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16270-1-PBS in a different storage buffer formulation.

  • Western Blot (WB) analysis of various lysates using PPCDC Polyclonal antibody (16270-1-AP)

    WB analysis using 16270-1-AP (same clone as 16270-1-PBS)

    Various lysates were subjected to SDS PAGE followed by western blot with 16270-1-AP (PPCDC antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16270-1-PBS in a different storage buffer formulation.

Informations sur le produit

16270-1-PBS cible PPCDC dans les applications de WB, Indirect ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène PPCDC Protéine recombinante Ag9337
Nom complet phosphopantothenoylcysteine decarboxylase
Masse moléculaire calculée 204 aa, 22 kDa
Poids moléculaire observé22-25 kDa, 14 kDa
Numéro d’acquisition GenBankBC014409
Symbole du gène PPCDC
Identification du gène (NCBI) 60490
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS only
Conditions de stockageStore at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Biosynthesis of coenzyme A (CoA) from pantothenic acid (vitamin B5) is an essential universal pathway in prokaryotes and eukaryotes. PPCDC, one of the last enzymes in this pathway, converts phosphopantothenoylcysteine to 4-prime-phosphopantetheine. The antibody was generated against full length human recombinant PPCDC, which is a bright yellow protein, indicating the presence of flavins. The antibody solution gives yellow color, too. It may be caused by elution of flavins during affinity purification with PPCDC-protein column.

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