Anticorps Polyclonal de lapin anti-PPDPF

PPDPF Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 19912-1-PBS

Synonymes

C20orf149, EXPDF

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Galerie de données de validation

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  WB analysis using 19912-1-AP (same clone as 19912-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse pancreas using 19912-1-AP (same clone as 19912-1-PBS)

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 19912-1-AP (PPDPF antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 19912-1-AP (same clone as 19912-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 19912-1-AP (C20orf149 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 19912-1-AP (same clone as 19912-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 10x lens). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  IHC staining of human pancreas using 19912-1-AP (same clone as 19912-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human pancreas using 19912-1-AP (PPDPF antibody) at dilution of 1:100 (under 40x lens). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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  IF Staining of U2OS using 19912-1-AP (same clone as 19912-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using PPDPF antibody (19912-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 19912-1-PBS in a different storage buffer formulation.

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Informations sur le produit

19912-1-PBS cible PPDPF dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPDPF Protéine recombinante Ag13740
• Nom complet: chromosome 20 open reading frame 149
• Masse moléculaire calculée: 114 aa, 12 kDa
• Poids moléculaire observé: 10-12 kDa
• Numéro d’acquisition GenBank: BC002531
• Symbole du gène: PPDPF
• Identification du gène (NCBI): 79144
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pancreatic progenitor cell differentiation and proliferation factor (PPDPF), also known as C20orf149 and EXPDF, belongs to the PPDPF family. PPDPF is a key regulator for the development of exocrine pancreas. PPDPF is highly expressed in various cancers, including HCC, colorectal cancer, prostate cancer, etc (PMID: 34031390, 34975328, 37027301). PPDPF consists of 114 amino acids and the molecular weight is 12 kDa.

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