Anticorps Polyclonal de lapin anti-PPFIA3

PPFIA3 Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 11492-1-AP

Synonymes

PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654

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Galerie de données de validation

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  WB analysis using 11492-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 11492-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11492-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 11492-1-AP

  IP result of anti-PPFIA3 (IP:11492-1-AP, 4ug; Detection:11492-1-AP 1:600) with mouse brain tissue lysate 3440ug.

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  IHC staining of mouse cerebellum using 11492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11492-1-AP (PPFIA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse cerebellum using 11492-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11492-1-AP (PPFIA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules HEK-293, tissu cérébral de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cervelet de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IHC: See 1 publications below

Informations sur le produit

11492-1-AP cible PPFIA3 dans les applications de WB, IHC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPFIA3 Protéine recombinante Ag1971
• Nom complet: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
• Masse moléculaire calculée: 1194 aa, 133 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC021255
• Symbole du gène: PPFIA3
• Identification du gène (NCBI): 8541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPFIA3, also named as KIAA0654, belongs to the liprin family and Liprin-alpha subfamily. PPFIA3 may regulate the disassembly of focal adhesions. It may localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. PPFIA3 exhibites more than 5 fold accumulations in the monoribosome fraction. PPFIA3 is decreased at the protein level by the miR-99 family but not in the luciferase reporter with the 3'UTR of PPFIA3. (PMID:21212412)

Publications

Species	Application	Title
human	WB	Cancer Res; miR-99 family of MicroRNAs suppresses the expression of prostate-specific antigen and prostate cancer cell proliferation.; Authors - Sun Dandan D
		Open Biol; The structural basis of the talin-KANK1 interaction that coordinates the actin and microtubule cytoskeletons at focal adhesions; Authors - Xingchen Li
mouse	IHC	Cell Rep; Neurexins play a crucial role in cerebellar granule cell survival by organizing autocrine machinery for neurotrophins.; Authors - Takeshi Uemura

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