Anticorps Polyclonal de lapin anti-PPFIA3

PPFIA3 Polyclonal Antibody for WB, IHC, IP, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IP, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 11492-1-PBS

Synonymes

PTPRF-interacting protein alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, Liprin-alpha-3, KIAA0654

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Galerie de données de validation

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  WB analysis using 11492-1-AP (same clone as 11492-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 11492-1-AP (same clone as 11492-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

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  WB analysis of HEK-293 using 11492-1-AP (same clone as 11492-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11492-1-AP (PPFIA3 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

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  IP experiment of mouse brain using 11492-1-AP (same clone as 11492-1-PBS)

  IP result of anti-PPFIA3 (IP:11492-1-AP, 4ug; Detection:11492-1-AP 1:600) with mouse brain tissue lysate 3440ug. This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 11492-1-AP (same clone as 11492-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11492-1-AP (PPFIA3 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 11492-1-AP (same clone as 11492-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 11492-1-AP (PPFIA3 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 11492-1-PBS in a different storage buffer formulation.

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Informations sur le produit

11492-1-PBS cible PPFIA3 dans les applications de WB, IHC, IP, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPFIA3 Protéine recombinante Ag1971
• Nom complet: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
• Masse moléculaire calculée: 1194 aa, 133 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC021255
• Symbole du gène: PPFIA3
• Identification du gène (NCBI): 8541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PPFIA3, also named as KIAA0654, belongs to the liprin family and Liprin-alpha subfamily. PPFIA3 may regulate the disassembly of focal adhesions. It may localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. PPFIA3 exhibites more than 5 fold accumulations in the monoribosome fraction. PPFIA3 is decreased at the protein level by the miR-99 family but not in the luciferase reporter with the 3'UTR of PPFIA3. (PMID:21212412)

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