Anticorps Recombinant de lapin anti-PPFIA3

• Phare
• Validé par KD/KO

PPFIA3 Recombinant Antibody for WB, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

CloneNo.

230459F6

N° de cat : 83142-4-RR

Synonymes

230459F6, KIAA0654, Liprin-alpha-3, Protein tyrosine phosphatase receptor type f polypeptide-interacting protein alpha-3, PTPRF-interacting protein alpha-3

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Galerie de données de validation

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  WB analysis using 83142-4-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 83142-4-RR (PPFIA3 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 83142-4-RR

  WB result of PPFIA3 antibody (83142-4-RR; 1:20000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PPFIA3 transfected HEK-293 cells.

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  IP experiment of mouse brain using 83142-4-RR

  IP result of anti-PPFIA3 (IP:83142-4-RR, 4ug; Detection:83142-4-RR 1:4000) with mouse brain tissue lysate 2100 ug.

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  FC experiment of HEK-293 using 83142-4-RR

  1x10^6 HEK-293 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  FC experiment of U-251 using 83142-4-RR

  1x10^6 U-251 cells were intracellularly stained with 0.25 ug PPFIA3 Recombinant antibody (83142-4-RR, Clone:230459F6) and APC-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L)(red), or 0.25 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 83142-4-RR

  Biolayer interferometry (BLl) kinetic assays of 83142-4-RR against Human PPFIA3 were performed. The affinity constant is 0.131 nM.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HEK-293, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en FC (Intra): cellules U-251, cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

83142-4-RR cible PPFIA3 dans les applications de WB, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PPFIA3 Protéine recombinante Ag1971
• Nom complet: protein tyrosine phosphatase, receptor type, f polypeptide (PTPRF), interacting protein (liprin), alpha 3
• Masse moléculaire calculée: 1194 aa, 133 kDa
• Poids moléculaire observé: 140 kDa
• Numéro d’acquisition GenBank: BC021255
• Symbole du gène: PPFIA3
• Identification du gène (NCBI): 8541
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPFIA3, also named as KIAA0654, belongs to the liprin family and Liprin-alpha subfamily. PPFIA3 may regulate the disassembly of focal adhesions. It may localize receptor-like tyrosine phosphatases type 2A at specific sites on the plasma membrane, possibly regulating their interaction with the extracellular environment and their association with substrates. PPFIA3 exhibites more than 5 fold accumulations in the monoribosome fraction. PPFIA3 is decreased at the protein level by the miR-99 family but not in the luciferase reporter with the 3'UTR of PPFIA3. (PMID:21212412)

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