Anticorps Polyclonal de lapin anti-Cyclophilin E

Cyclophilin E Polyclonal Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

Avis (3)

N° de cat : 15357-1-AP

Synonymes

PPIE, Cyclophilin 33, Cyclophilin-33, CYP 33, CYP33

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Galerie de données de validation

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  WB analysis using 15357-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15357-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15357-1-AP (Cyclophilin E antibody at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  IF Staining of HeLa using 15357-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cyclophilin E antibody (15357-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules BxPC-3, cellules HeLa, cellules Jurkat
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:5000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

15357-1-AP cible Cyclophilin E dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cyclophilin E Protéine recombinante Ag7603
• Nom complet: peptidylprolyl isomerase E (cyclophilin E)
• Masse moléculaire calculée: 33 kDa
• Poids moléculaire observé: 33-38 kDa
• Numéro d’acquisition GenBank: BC004898
• Symbole du gène: Cyclophilin E
• Identification du gène (NCBI): 10450
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cyclophilin E (CypE), also known as Cyclophilin-33, Rotamase E, CYP33, PPIE, is an enzyme which belongs to the cyclophilin-type PPIase family of PPIase E subfamily (PMID: 15998457). Cyclophilin E negatively regulates influenza virus replication and transcription occurs by impairing the formation of the vRNP (PMID: 21887220). Additionally, Cyclophilin E plays an important role in osteoblast differentiation as a positive regulator by increasing the transcriptional activity of Runx2 (PMID: 37947627).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Aleksandr (Verified Customer) (07-19-2025)

Overall antibody recognize the band of the expected size, but there are several non-specific bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: RKO

Cyclophilin E Antibody Western Blot validation (1:2000 dilution) in RKO (Cat no:15357-1-AP)

FH

Moritz (Verified Customer) (09-12-2024)

Several bands were observable in WB, also the expected size of PPIE, although it was not the strongest.

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T

Cyclophilin E Antibody Western Blot validation (1:3000 dilution) in HEK293T (Cat no:15357-1-AP)

FH

Tsimafei (Verified Customer) (08-12-2024)

Multiple bands are present, however the one corresponding to Cyclophilin E size (~33 kDa) is clearly visible

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: mESCs

Cyclophilin E Antibody Western Blot validation (1:3000 dilution) in mESCs (Cat no:15357-1-AP)

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