Anticorps Monoclonal anti-PPM1B

PPM1B Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(2)

CloneNo.

2C7H2

N° de cat : 67647-1-Ig

Synonymes

2C7H2, EC:3.1.3.16, PP2C beta, PP2CB, PP2C-beta

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Galerie de données de validation

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  WB analysis using 67647-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 67647-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of human breast cancer using 67647-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human breast cancer using 67647-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 67647-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse skeletal muscle using 67647-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A431 using 67647-1-Ig

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPM1B antibody (67647-1-Ig, Clone: 2C7H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1).

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  Affinity and Kinetic Characterization of 67647-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 67647-1-Ig against Human PPM1B was performed. The affinity constant is below 1 pM.

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules HepG2, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules NIH/3T3
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de muscle squelettique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IF: See 1 publications below

Informations sur le produit

67647-1-Ig cible PPM1B dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: singe
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPM1B Protéine recombinante Ag3871
• Nom complet: protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
• Masse moléculaire calculée: 479 aa, 53 kDa
• Poids moléculaire observé: 36, 53 kDa
• Numéro d’acquisition GenBank: BC012002
• Symbole du gène: PPM1B
• Identification du gène (NCBI): 5495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.

Publications

Species	Application	Title
		bioRxiv; Epigenetic modulation of NLRP6 inflammasome sensor as a therapeutic modality to reduce necroptosis-driven gastrointestinal mucosal dysfunction in HIV/SIV infection; Authors - Lakmini S Premadasa
monkey	IF	Cell Commun Signal; Epigenetic modulation of the NLRP6 inflammasome sensor as a therapeutic modality to reduce necroptosis-driven gastrointestinal mucosal dysfunction in HIV/SIV infection; Authors - Lakmini S Premadasa

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