Anticorps Monoclonal anti-PPM1B

PPM1B Monoclonal Antibody for WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test

Conjugaison

Non conjugué

CloneNo.

2C7H2

N° de cat : 67647-1-PBS

Synonymes

2C7H2, EC:3.1.3.16, PP2C beta, PP2CB, PP2C-beta

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Galerie de données de validation

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  WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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  WB analysis using 67647-1-Ig (same clone as 67647-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67647-1-Ig (PPM1B antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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  Sample test of MP51322-1

  Sample test of MP51322-1, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-2-PBS.

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  Sample test of MP51322-2

  Sample test of MP51322-2, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-3-PBS.

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  Sample test of MP51322-3

  Sample test of MP51322-3, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-4-PBS.

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  Sample test of MP51322-4

  Sample test of MP51322-4, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-5-PBS.

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  IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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  IHC staining of human breast cancer using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 67647-1-Ig (PPM1B antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 67647-1-Ig (same clone as 67647-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPM1B antibody (67647-1-Ig, Clone: 2C7H2 ) at dilution of 1:1000 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67647-1-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP51322-4

  Cytometric bead array standard curve of MP51322-4, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-5-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

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  Cytometric bead array standard curve of MP51322-1

  Cytometric bead array standard curve of MP51322-1, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-2-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

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  Cytometric bead array standard curve of MP51322-2

  Cytometric bead array standard curve of MP51322-2, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-3-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

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  Cytometric bead array standard curve of MP51322-3

  Cytometric bead array standard curve of MP51322-3, PPM1B Monoclonal Matched Antibody Pair, PBS Only. Capture antibody: 67647-1-PBS. Detection antibody: 67647-4-PBS. Standard:Ag3871. Range: 0.195-200 ng/mL.

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  Affinity and Kinetic Characterization of 67647-1-Ig

  Biolayer interferometry (BLl) kinetic assay of 67647-1-Ig against Human PPM1B was performed. The affinity constant is below 1 pM.

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Informations sur le produit

67647-1-PBS cible PPM1B dans les applications de WB, IHC, IF/ICC, Cytometric bead array, Indirect ELISA, Sample test et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPM1B Protéine recombinante Ag3871
• Nom complet: protein phosphatase 1B (formerly 2C), magnesium-dependent, beta isoform
• Masse moléculaire calculée: 479 aa, 53 kDa
• Poids moléculaire observé: 36, 53 kDa
• Numéro d’acquisition GenBank: BC012002
• Symbole du gène: PPM1B
• Identification du gène (NCBI): 5495
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PPM1B can identify 4 isoforms with the WM of 36-55 kDa.

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