Anticorps Monoclonal anti-PPM1D

PPM1D Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2D6D5

N° de cat : 67440-1-PBS

Synonymes

PP2C DELTA, PPM1D, Protein phosphatase 1D, WIP1

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Galerie de données de validation

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  WB analysis of MCF-7 using 67440-1-Ig (same clone as 67440-1-PBS)

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

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  WB analysis of pig brain using 67440-1-Ig (same clone as 67440-1-PBS)

  pig brain tissue were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

• 

  WB analysis of rat brain using 67440-1-Ig (same clone as 67440-1-PBS)

  rat brain tissue were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

• 

  WB analysis of mouse brain using 67440-1-Ig (same clone as 67440-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

• 

  WB analysis of HEK-293 using 67440-1-Ig (same clone as 67440-1-PBS)

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

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  WB analysis of U2OS using 67440-1-Ig (same clone as 67440-1-PBS)

  U2OS cells were subjected to SDS PAGE followed by western blot with 67440-1-Ig (PPM1D antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

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  IHC staining of human colon cancer using 67440-1-Ig (same clone as 67440-1-PBS)

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 67440-1-Ig (PPM1D antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

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  IF Staining of HepG2 using 67440-1-Ig (same clone as 67440-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using 67440-1-Ig (PPM1D antibody), at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 67440-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67440-1-PBS cible PPM1D dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPM1D Protéine recombinante Ag24761
• Nom complet: protein phosphatase 1D magnesium-dependent, delta isoform
• Poids moléculaire observé: 67 kDa, 47 kDa
• Numéro d’acquisition GenBank: BC060877
• Symbole du gène: PPM1D
• Identification du gène (NCBI): 8493
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PPM1D (also known as PP2Cd and WIP1) is a member of the PPM family, which is characterized by Mg2+/Mn2+-dependent activity and relative insensitivity to okadaic acid. The PPM1D gene is aberrantly amplified in a range of common cancers and encodes a protein phosphatase that is a potential therapeutic target (PMID: 17700519). This antibody can be detected two bands of 47 and 67 Kda.

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