Anticorps Polyclonal de lapin anti-PPM1E

PPM1E Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 25089-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 25089-1-AP (same clone as 25089-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 25089-1-AP (PPM1E antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

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  IHC staining of rat brain using 25089-1-AP (same clone as 25089-1-PBS)

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 25089-1-AP (PPM1E antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 25089-1-AP (same clone as 25089-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 25089-1-AP (same clone as 25089-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPM1E antibody (25089-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 25089-1-PBS in a different storage buffer formulation.

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Informations sur le produit

25089-1-PBS cible PPM1E dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPM1E Protéine recombinante Ag18751
• Nom complet: protein phosphatase 1E (PP2C domain containing)
• Masse moléculaire calculée: 764 aa, 85 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC136290
• Symbole du gène: PPM1E
• Identification du gène (NCBI): 22843
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Ca2+/calmodulin-dependent protein kinase phosphatase (CaMKP-N),also known as Ppm1E, is an AMPKα phosphatase,which is up-regulated in human gastric cancer tissues (PMID: 28423719).

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