Anticorps Monoclonal anti-PPM1F

PPM1F Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

CloneNo.

2A2E8

N° de cat : 68626-1-Ig

Synonymes

CaM kinase phosphatase, CaMKPase, FEM 2, hFEM 2, KIAA0015, Partner of PIX 2, POPX2, PPM1F, Protein fem 2 homolog, Protein phosphatase 1F

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Galerie de données de validation

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  WB analysis using 68626-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control.

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  WB analysis using 68626-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 68626-1-Ig (PPM1F antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U2OS, cellules A375, cellules HEK-293, cellules HepG2, cellules Jurkat, cellules K-562, plaquettes de sang périphérique humain

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

68626-1-Ig cible PPM1F dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPM1F Protéine recombinante Ag10316
• Nom complet: protein phosphatase 1F (PP2C domain containing)
• Masse moléculaire calculée: 453 aa, 50 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC071989
• Symbole du gène: PPM1F
• Identification du gène (NCBI): 9647
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mg2+/Mn2+-dependent protein phosphatase 1F (PPM1F) belongs to the PP2C family of Ser/Thr protein phosphatases and is the critical enzyme responsible for dephosphorylating the threonine motif. PPM1F is ubiquitous in various tissues and organs (PMID: 26498692). High expression of PPM1F was positively associated with poor survival and tumor recurrence in patients with cancers and PPM1F might be a potential biomarker in HCC patients (PMID: 30470261).

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