Anticorps Recombinant de lapin anti-PDP1

• Phare
• Validé par KD/KO

PDP1 Recombinant Antibody for WB, IHC, Cytometric bead array, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, Cytometric bead array, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242090E7

N° de cat : 84612-3-PBS

Synonymes

PPM2C, [Pyruvate dehydrogenase [acetyl-transferring]]-phosphatase 1, mitochondrial, 242090E7, EC:3.1.3.43, PDP 1

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Galerie de données de validation

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  WB analysis of PC-3 using 84612-3-RR (same clone as 84612-3-PBS)

  WB result of PDP1 antibody (84612-3-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-PDP1 transfected PC-3 cells. This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

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  WB analysis using 84612-3-RR (same clone as 84612-3-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 84612-3-RR (PDP1 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

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  IHC staining of human prostate cancer using 84612-3-RR (same clone as 84612-3-PBS)

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 84612-3-RR (PDP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

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  IHC staining of mouse skeletal muscle using 84612-3-RR (same clone as 84612-3-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 84612-3-RR (PDP1 antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 84612-3-PBS in a different storage buffer formulation.

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  Cytometric bead array standard curve of MP01450-2

  Cytometric bead array standard curve of MP01450-2, PPM2C Recombinant Matched Antibody Pair, PBS Only. Capture antibody: 84612-1-PBS. Detection antibody: 84612-3-PBS. Standard: Ag15453. Range: 2.5-80 ng/mL.

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  Affinity and Kinetic Characterization of 84612-3-PBS

  Biolayer interferometry (BLl) kinetic assay of 84612-3-PBS against Human PDP1 was performed. The affinity constant is 358 nM.

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  Affinity and Kinetic Characterization of 84612-3-RR

  Biolayer interferometry (BLl) kinetic assays of 84612-3-RR against Human PDP1 were performed. The affinity constant is below 1 pM.

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Informations sur le produit

84612-3-PBS cible PDP1 dans les applications de WB, IHC, Cytometric bead array, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PDP1 Protéine recombinante Ag15453
• Nom complet: protein phosphatase 2C, magnesium-dependent, catalytic subunit
• Masse moléculaire calculée: 537 aa, 61 kDa
• Poids moléculaire observé: 55-61 kDa
• Numéro d’acquisition GenBank: BC047619
• Symbole du gène: PDP1
• Identification du gène (NCBI): 54704
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Pyruvate dehydrogenase phosphatase 1 (PDP1), also known as PPM2C, is a key hormone-regulated metabolic enzyme that dephosphorylates and activates pyruvate dehydrogenase (PDH), thereby stimulating the conversion of pyruvate into acetyl-CoA (PMID: 36453802). PDP1 expression is regulated by FLT3-ITD in a context-dependent manner, and that it is centrally involved in the adaptation of AML glucose metabolism to the requirements of proliferation, survival and the therapeutic response to FLT3 inhibition (PMID: 37935978).

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