Anticorps Polyclonal de lapin anti-PPME1

PPME1 Polyclonal Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 14435-1-PBS

Synonymes

EC:3.1.1.89, PME 1, PME1, PME-1, PP2593

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Galerie de données de validation

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  WB analysis using 14435-1-AP (same clone as 14435-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of human brain using 14435-1-AP (same clone as 14435-1-PBS)

  human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of fetal human brain using 14435-1-AP (same clone as 14435-1-PBS)

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of mouse brain using 14435-1-AP (same clone as 14435-1-PBS)

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of A549 using 14435-1-AP (same clone as 14435-1-PBS)

  A549 cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  WB analysis of HeLa using 14435-1-AP (same clone as 14435-1-PBS)

  HeLa cells were subjected to SDS PAGE followed by western blot with 14435-1-AP (PPME1 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 14435-1-AP (same clone as 14435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14435-1-AP (same clone as 14435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse testis using 14435-1-AP (same clone as 14435-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 14435-1-AP (PPME1 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 14435-1-PBS in a different storage buffer formulation.

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Informations sur le produit

14435-1-PBS cible PPME1 dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPME1 Protéine recombinante Ag5817
• Nom complet: protein phosphatase methylesterase 1
• Masse moléculaire calculée: 42 kDa
• Poids moléculaire observé: 44 kDa
• Numéro d’acquisition GenBank: BC050705
• Symbole du gène: PPME1
• Identification du gène (NCBI): 51400
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PPME1 (Protein phosphatase methylesterase 1) is a protein phosphatase 2A (PP2A)-specific methyl esterase that negatively regulates PP2A through demethylation at its carboxy terminal leucine 309 residue (PMID: 24253382). PPME1 plays an important role in the development of cancers and may be a biomarker for pancreatic carcinoma, gastric and lung cancer (PMID: 32351291).

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