Anticorps Recombinant de lapin anti-PPP1CB

PPP1CB Recombinant Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

CloneNo.

242547H7

N° de cat : 84938-5-RR

Synonymes

242547H7, EC:3.1.3.16, PP 1B, PP1 Beta, PP-1B

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Galerie de données de validation

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  WB analysis using 84938-5-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 84938-5-RR (PPP1CB antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 84938-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84938-5-RR (PPP1CB antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 84938-5-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 84938-5-RR (PPP1CB antibody) at dilution of 1:500 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of A549 using 84938-5-RR

  Immunofluorescent analysis of (4% PFA) fixed A549 cells using PPP1CB antibody (84938-5-RR, Clone: 242547H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  IF Staining of MCF-7 using 84938-5-RR

  Immunofluorescent analysis of (4% PFA) fixed MCF-7 cells using PPP1CB antibody (84938-5-RR, Clone: 242547H7 ) at dilution of 1:500 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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  FC experiment of Jurkat using 84938-5-RR

  1x10^6 Jurkat cells were intracellularly stained with 0.25 ug PPP1CB Recombinant antibody (84938-5-RR, Clone:242547H7) and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.25 ug Rabbit IgG Isotype Control RecAb (98136-1-RR, Clone: 240953C9) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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  Affinity and Kinetic Characterization of 84938-5-RR

  Biolayer interferometry (BLl) kinetic assays of 84938-5-RR against Human PPP1CB were performed. The affinity constant is 2.44 nM.

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules Jurkat, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules MCF-7, cellules A549
• Résultats positifs en FC (Intra): cellules Jurkat,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:250-1:1000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.25 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

84938-5-RR cible PPP1CB dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PPP1CB Protéine recombinante Ag0183
• Nom complet: protein phosphatase 1, catalytic subunit, beta isoform
• Masse moléculaire calculée: 37 kDa
• Poids moléculaire observé: 35-37 kDa
• Numéro d’acquisition GenBank: BC002697
• Symbole du gène: PPP1CB
• Identification du gène (NCBI): 5500
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase 1 (PP1) is the main eukaryotic protein serine/threonine phosphatase that regulates an enormous variety of cellular processes including protein-protein interactions, gene transcription, cell-cycle progression and apoptosis. It is a trimeric complex composed of a regulatory subunit, a variable subunit and a catalytic subunit. The catalytic subunit of PP1 (PP1c) can interact with amount of regulatory subunits in vivo that target it to particular subcellular locations and regulate its activity towards specific substrates.

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