Anticorps Monoclonal anti-PPP1CC

PPP1CC Monoclonal Antibody for WB, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2G11C3

N° de cat : 68122-1-PBS

Synonymes

2G11C3, EC:3.1.3.16, PP-1G, Serine/threonine-protein phosphatase PP1-gamma catalytic subunit

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Galerie de données de validation

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  WB analysis using 68122-1-Ig (same clone as 68122-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 68122-1-Ig (PPP1CC antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated Beta Actin Monoclonal antibody (HRP-66009) as loading control. This data was developed using the same antibody clone with 68122-1-PBS in a different storage buffer formulation.

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  IF Staining of C2C12 using 68122-1-Ig (same clone as 68122-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L). This data was developed using the same antibody clone with 68122-1-PBS in a different storage buffer formulation.

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  IF Staining of HeLa using 68122-1-Ig (same clone as 68122-1-PBS)

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using PPP1CC antibody (68122-1-Ig, Clone: 2G11C3 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L), CL594-Phalloidin (red). This data was developed using the same antibody clone with 68122-1-PBS in a different storage buffer formulation.

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Informations sur le produit

68122-1-PBS cible PPP1CC dans les applications de WB, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPP1CC Protéine recombinante Ag32348
• Nom complet: protein phosphatase 1, catalytic subunit, gamma isoform
• Masse moléculaire calculée: 35 kDa
• Poids moléculaire observé: 35 kDa
• Numéro d’acquisition GenBank: BC014073
• Symbole du gène: PPP1CC
• Identification du gène (NCBI): 5501
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

PPP1CC, also named as PP-1G, belongs to the PPP phosphatase family and PP-1 subfamily. Protein phosphatase 1 (PP1) is essential for cell division, and participates in the regulation of glycogen metabolism, muscle contractility and protein synthesis. It is involved in regulation of ionic conductances and long-term synaptic plasticity. PPP1CC may play an important role in dephosphorylating substrates such as the postsynaptic density-associated Ca2+/calmodulin dependent protein kinase II.

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