Anticorps Recombinant de lapin anti-PPP1R13L

PPP1R13L Recombinant Antibody for WB, Sandwich ELISA, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, Sandwich ELISA, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

250633E12

N° de cat : 86044-1-PBS

Synonymes

IASPP, Inhibitor of ASPP protein, NFkB-interacting protein 1, NKIP1, PPP1R13B like protein

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Galerie de données de validation

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  WB analysis using 86044-1-RR (same clone as 86044-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 86044-1-RR (PPP1R13L antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 86044-1-PBS in a different storage buffer formulation.

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  Sandwich ELISA sample test of MP02334-1

  The mean iASPP concentration was determined to be 32.51 ng/mL in PC-3 cell extract based on a 2.0 mg/mL extract load and 68.98 ng/mL in MCF-7 cell extract based on a 1.3 mg/mL extract load.

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  Sandwich ELISA standard curve of MP02334-1

  Sandwich ELISA standard curve of MP02334-1, Human iASPP Recombinant Matched Antibody Pair - PBS only. 86044-3-PBS was coated to a plate as the capture antibody and incubated with serial dilutions of standard Ag13273. 86044-1-PBS was HRP conjugated as the detection antibody. Range: 0.313-20 ng/mL

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  Affinity and Kinetic Characterization of 86044-1-RR

  Biolayer interferometry (BLl) kinetic assays of 86044-1-RR against Human PPP1R13L were performed. The affinity constant is below 1 pM.

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Informations sur le produit

86044-1-PBS cible PPP1R13L dans les applications de WB, Sandwich ELISA, Indirect ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PPP1R13L Protéine recombinante Ag13273
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 13 like
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 110 kDa
• Numéro d’acquisition GenBank: BC064913
• Symbole du gène: PPP1R13L
• Identification du gène (NCBI): 10848
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Inhibitor of apoptosis-stimulating protein of p53 (iASPP), encoded by PPP1R13L gene, is often overexpressed in human cancers. The ASPP family includes three members, namely ASPP1, ASPP2, and iASPP, which are specific regulators of p53-, p63-, and p73-mediated apoptosis. ASPP1 and ASPP2 enhance the apoptotic function of p53, whereas iASPP specifically inhibits p53-mediated apoptosis. Overexpression of iASPP is associated with resistance to cisplatin-induced apoptosis and rediation therapy. iASPP plays a pivotal role in regulating cancer cell proliferation and tumor progression.

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