• Phare
  • Validé par KD/KO

Anticorps Polyclonal de lapin anti-GADD34

GADD34 Polyclonal Antibody for WB, IP, IF, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (4)

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

N° de cat : 10449-1-AP

Synonymes

GADD34, growth arrest and DNA damage-inducible protein 34, PPP1R15A



Applications testées

Résultats positifs en WBcellules NIH/3T3 traitées au MG132, cellules HeLa traitées à la tunicamycine, cellules HepG2, cellules Jurkat, cellules K-562, cellules PC-3
Résultats positifs en IPcellules K-562, cellules NIH/3T3
Résultats positifs en IHCtissu pancréatique humain, tissu de cancer du côlon humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
Résultats positifs en IF/ICCcellules PC-3

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:1000-1:6000
Immunoprécipitation (IP)IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
Immunohistochimie (IHC)IHC : 1:50-1:500
Immunofluorescence (IF)/ICCIF/ICC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

10449-1-AP cible GADD34 dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, souris

Réactivité Humain, souris
Réactivité citéerat, Humain, porc, singe, souris, Hamster
Hôte / Isotype Lapin / IgG
Clonalité Polyclonal
Type Anticorps
Immunogène GADD34 Protéine recombinante Ag0578
Nom complet protein phosphatase 1, regulatory (inhibitor) subunit 15A
Masse moléculaire calculée 73 kDa
Poids moléculaire observé 100 kDa
Numéro d’acquisition GenBankBC003067
Symbole du gène GADD34
Identification du gène (NCBI) 23645
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par affinité contre l'antigène
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

GADD34, also named PPP1R15A, belongs to the PPP1R15 family. GADD34 can be triggered as a direct target of activating transcription factor4 (ATF4) under ER stress, it plays a pivotal role in the recovery of cells from shut-down of translation induced by ER stress. It recruits the serine/threonine-protein phosphatase (PP1) to dephosphorylate the translation initiation factor eIF2alpha, thereby reversing the shut-off of protein synthesis initiated by stress-inducible kinases and facilitating recovery of cells from stress. GADD34 down-regulates the TGF-beta signaling pathway by promoting dephosphorylation of TGFB1 via PP1. It may also promote apoptosis by inducing TP53 phosphorylation on 'Ser-15'. Starvation-induced expression of GADD34 reduced mTOR activity and induced autophagy in wild-type mice, but not in GADD34 KO mice. Molecular weight of GADD34 is 100 kDa confirmed in GADD34 KO mice, and Proteintech's GADD34 antibody 10449-1-AP primarily recongize the 100 kDa band.

Protocole

Product Specific Protocols
WB protocol for GADD34 antibody 10449-1-APDownload protocol
IHC protocol for GADD34 antibody 10449-1-APDownload protocol
IF protocol for GADD34 antibody 10449-1-APDownload protocol
IP protocol for GADD34 antibody 10449-1-APDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
WB

Nature

Stress response silencing by an E3 ligase mutated in neurodegeneration

Authors - Diane L Haakonsen
humanWB

Nature

An inhibitor of NEDD8-activating enzyme as a new approach to treat cancer.

Authors - Soucy Teresa A TA
mouseWB,IHC

Nature

Sustained translational repression by eIF2α-P mediates prion neurodegeneration.

Authors - Moreno Julie A JA
humanWB

Science

Preventing proteostasis diseases by selective inhibition of a phosphatase regulatory subunit.

Authors - Indrajit Das
humanWB

Cell

Target-Based Discovery of an Inhibitor of the Regulatory Phosphatase PPP1R15B.

Authors - Agnieszka Krzyzosiak
humanWB, IP

Science

Selective inhibition of a regulatory subunit of protein phosphatase 1 restores proteostasis.

Authors - Pavel Tsaytler

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.


FH

Ana (Verified Customer) (03-11-2021)

GADD34 in mouse muscle. 10 ug of protein. BSA blocking. 1:1000 primary ab dilution O/N incubation 4ºC.1:5000 secondary ab HRP conjugated.

  • Applications: Western Blot
  • Primary Antibody Dilution: 1:1000
  • Cell Tissue Type: Mouse Muscle
GADD34 Antibody Western Blot validation (1:1000 dilution) in Mouse Muscle (Cat no:10449-1-AP)
FH

Maria (Verified Customer) (02-10-2021)

GADD34 in human primary fibroblasts. UT and TN treated. PFA 4% 20 min. Blocking: PBST (0.1% Triton) 10% Goat Serum 1h RT. Primary incubation: 16h 4ºC 1:200 in PBST 10% GS. Secondary: 2h RT 1:500 Goat anti-rabbit Alexa fluor 555 in PBST 10% GS.

  • Applications: Immunofluorescence
  • Primary Antibody Dilution: 1:200
  • Cell Tissue Type: Human primary fibroblasts
GADD34 Antibody Immunofluorescence validation (1:200 dilution) in Human primary fibroblasts (Cat no:10449-1-AP)
FH

Daniel (Verified Customer) (10-01-2019)

Cytoplasmic staining of the tubules of wild-type kidneys.

  • Applications: Immunohistochemistry,
  • Primary Antibody Dilution: 1:100
  • Cell Tissue Type: Mouse kidney
GADD34 Antibody Immunohistochemistry, validation (1:100 dilution) in Mouse kidney (Cat no:10449-1-AP)
FH

Stefan (Verified Customer) (07-31-2019)

See publication:Dalton LE, Clarke HJ, Knight J, Lawson MH, Wason J, Lomas DA, Howat WJ, Rintoul RC, Rassl DM, Marciniak SJ. (2013). The endoplasmic reticulum stress marker CHOP predicts survival in malignant mesothelioma. Br J Cancer. 2013 Apr 2;108(6):1340-7. doi: 10.1038/bjc.2013.66. Epub 2013 Feb 14.https://www.nature.com/articles/bjc201366

  • Applications: Western Blot, Immunohistochemistry,
  • Primary Antibody Dilution: WB 1:1000, IHC 1:100
  • Cell Tissue Type: HCT116 human colon carcinoma
GADD34 Antibody Western Blot,Immunohistochemistry, validation (WB 1:1000, IHC 1:100 dilution) in HCT116 human colon carcinoma (Cat no:10449-1-AP)