Anticorps Recombinant de lapin anti-PPP1R15B

• Phare
• Validé par KD/KO

PPP1R15B Recombinant Antibody for IF/ICC, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

IF/ICC, FC (Intra)

Conjugaison

CoraLite® Plus 488 Fluorescent Dye

CloneNo.

240052G5

N° de cat : CL488-83016-6

Synonymes

PPP1R15B;CReP, 240052G5, CREP, FLJ14744

---

Galerie de données de validation

• 

  IF Staining of HepG2 using CL488-83016-6

  Immunofluorescent analysis of (-20°C Ethanol) fixed HepG2 cells using CoraLite® Plus 488 PPP1R15B antibody (CL488-83016-6, Clone: 240052G5 ) at dilution of 1:200.

• 

  FC experiment of U-2 OS using CL488-83016-6

  1x10^6 U-2 OS were intracellularly stained with 0.4 ug Coralite® Plus 488 Ppp1r15b Recombinant Antibody (CL488-83016-6, Clone:240052G5), and 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

• 

  FC experiment of HepG2 using CL488-83016-6

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug Coralite® Plus 488 Ppp1r15b Recombinant Antibody (CL488-83016-6, Clone:240052G5)(red), or 0.4 ug CoraLite® Plus 488-conjugated Rabbit IgG control Rabbit PolyAb (CL488-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): U-2 OS, cellules HepG2

Dilution recommandée

• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL488-83016-6 cible PPP1R15B dans les applications de IF/ICC, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: PPP1R15B Protéine recombinante Ag6213
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 15B
• Masse moléculaire calculée: 79 kDa
• Poids moléculaire observé: 100-110 kDa
• Numéro d’acquisition GenBank: BC065280
• Symbole du gène: PPP1R15B
• Identification du gène (NCBI): 84919
• Conjugaison: CoraLite® Plus 488 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 493 nm / 522 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Protein phosphatase 1 regulatory subunit 15B(PPP1R15B) is a member of PPP1R15 family, it forms a complex with protein phosphatase1(PP1) and NCK1/2, maintaining low levels of EIF2S1 phosphorylation in unstressed cells, thus is also named constitutive repressor of eIF2alpha phosphorylation (CReP). PPP1R15B is assoicated in endoplasmic reticulum (ER) stress response resulting to a role in translation regulation. Knock-down of PPP1R15B strongly protected mammalian cells against oxidative stress, peroxynitrite stress, and more modestly against accumulation of malfolded proteins in the ER. Recently, PPP1R15B was found to associate with cell membranes and regulate membrane traffic in a PP1c-independent manner, suggesting a novel link between translation and traffic. MW of PPP1R15B is 80 kDa. Observed MW of PPP1R15B is from 100-110 kDa maybe due to phosphorylation (PMID: 28492545).

{{ptg:RelatedPrimaryAntibodies}}