Anticorps Polyclonal de lapin anti-DARPP32

DARPP32 Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, IF-P, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 10748-1-AP

Synonymes

DARPP32/PPP1R1B, PPP1R1B, Dopamine- and cAMP-regulated neuronal phosphoprotein, DARPP-32

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Galerie de données de validation

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  WB analysis using 10748-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 10748-1-AP (DARPP32 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 10748-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 10748-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat brain using 10748-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat brain using 10748-1-AP

  Immunohistochemical analysis of paraffin-embedded rat brain tissue slide using 10748-1-AP (DARPP32 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse brain using 10748-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using DARPP32/PPP1R1B antibody (10748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of SH-SY5Y using 10748-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using DARPP32/PPP1R1B antibody (10748-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, tissu cérébral de rat
• Résultats positifs en IHC: tissu cérébral de souris, tissu cérébral de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu cérébral de souris,
• Résultats positifs en IF/ICC: cellules SH-SY5Y,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

10748-1-AP cible DARPP32 dans les applications de WB, IHC, IF/ICC, IF-P, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: DARPP32 Protéine recombinante Ag1216
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 1B
• Masse moléculaire calculée: 23 kDa
• Poids moléculaire observé: 32 kDa
• Numéro d’acquisition GenBank: BC001519
• Symbole du gène: DARPP32
• Identification du gène (NCBI): 84152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Dopamine- and cAMP-regulated neuronal phosphoprotein (DARPP32) , also known as protein phosphatase 1 regulatory subunit 1B (PPP1R1B), is a substrate of cAMP-dependent protein kinase (PKA) enriched in dopamine-innervated brain areas. Overexpression of DARPP32 has been shown to block gefitinib-induced apoptosis in gastric tumors by promoting EGFR and ERBB3 heterodimerization (PMID: 21741919). DARPP32 also drives gefitinib-refractory lung tumor growth through similar ERBB3-mediated resistance mechanisms(PMID: 34675407). The molecular weight of DARPP32 is about 32 kDa.

Publications

Species	Application	Title
mouse	WB	Acta Pharmacol Sin; Ginsenoside Rg1 exerts neuroprotective effects in 3-nitropronpionic acid-induced mouse model of Huntington's disease via suppressing MAPKs and NF-κB pathways in the striatum.; Authors - Xiong Yang
mouse	IF	CNS Neurosci Ther; A New VMAT-2 Inhibitor NBI-641449 in the Treatment of Huntington Disease.; Authors - Sheng Chen
mouse	WB	Free Radic Biol Med; Disruption of Cdk5-GluN2B complex by a small interfering peptide attenuates social isolation-induced escalated intermale attack behavior and hippocampal oxidative stress in mice; Authors - Heng Ai
mouse	IF	Transl Psychiatry; Exploring PDE5A upregulation in bipolar disorder: insights from single-nucleus RNA sequencing of human basal ganglia; Authors - Zhixin Bai

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