Anticorps Recombinant de lapin anti-PPP1R9B

PPP1R9B Recombinant Antibody for WB, IHC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

242418G10

N° de cat : 85198-1-PBS

Synonymes

Neurabin 2, 242418G10, Neurabin II, Neurabin-2, Neurabin-II

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Galerie de données de validation

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  WB analysis using 85198-1-RR (same clone as 85198-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85198-1-RR (PPP1R9B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85198-1-PBS in a different storage buffer formulation.

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  WB analysis using 85198-1-RR (same clone as 85198-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 85198-1-RR (PPP1R9B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 85198-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 85198-1-RR (same clone as 85198-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85198-1-RR (PPP1R9B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 85198-1-PBS in a different storage buffer formulation.

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Informations sur le produit

85198-1-PBS cible PPP1R9B dans les applications de WB, IHC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 9B
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_032595
• Symbole du gène: Spinophilin/Neurabin 2
• Identification du gène (NCBI): 84687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

Neurabin 2, also named as Spinophilin, seems to act as a scaffold protein in multiple signaling pathways. It modulates excitatory synaptic transmission and dendritic spine morphology. PPP1R9B binds to actin filaments (F-actin) and shows cross-linking activity. It may play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. PPP1R9B plays a role in regulation of G-protein coupled receptor signaling, including D2Rs and alpha-adrenergic receptors. PPP1R9B probably regulates p70 S6 kinase activity by forming a complex with TIAM. The antibody is specific to PPP1R9B. The predicted molecular weight of spinophilin is 89 kDa, which differs significantly from the apparent MW seen in SDS/PAGE. Both the expressed full-length cDNA and the endogenous protein run at 120-130 kDa. This may be due to an extended conformation and/or low SDS binding capacity. (PMID: 28941770, PMID: 9275233).

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