Anticorps Recombinant de lapin anti-PPP1R9B

PPP1R9B Recombinant Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

242418G10

N° de cat : 85198-1-RR

Synonymes

Neurabin 2, 242418G10, Neurabin II, Neurabin-2, Neurabin-II

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Galerie de données de validation

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  WB analysis using 85198-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85198-1-RR (PPP1R9B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 85198-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 85198-1-RR (PPP1R9B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 85198-1-RR

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 85198-1-RR (PPP1R9B antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cérébral de souris, cellules A549, cellules MCF-7, tissu cérébral de rat, tissu de cervelet de souris
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

85198-1-RR cible PPP1R9B dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: protein phosphatase 1, regulatory (inhibitor) subunit 9B
• Masse moléculaire calculée: 89 kDa
• Poids moléculaire observé: 120-130 kDa
• Numéro d’acquisition GenBank: NM_032595
• Symbole du gène: Spinophilin/Neurabin 2
• Identification du gène (NCBI): 84687
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Protein A purfication
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Neurabin 2, also named as Spinophilin, seems to act as a scaffold protein in multiple signaling pathways. It modulates excitatory synaptic transmission and dendritic spine morphology. PPP1R9B binds to actin filaments (F-actin) and shows cross-linking activity. It may play an important role in linking the actin cytoskeleton to the plasma membrane at the synaptic junction. PPP1R9B plays a role in regulation of G-protein coupled receptor signaling, including D2Rs and alpha-adrenergic receptors. PPP1R9B probably regulates p70 S6 kinase activity by forming a complex with TIAM. The antibody is specific to PPP1R9B. The predicted molecular weight of spinophilin is 89 kDa, which differs significantly from the apparent MW seen in SDS/PAGE. Both the expressed full-length cDNA and the endogenous protein run at 120-130 kDa. This may be due to an extended conformation and/or low SDS binding capacity. (PMID: 28941770, PMID: 9275233).

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