Anticorps Polyclonal de lapin anti-PPP2R2A

PPP2R2A Polyclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

N° de cat : 16569-1-PBS

Synonymes

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Galerie de données de validation

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  WB analysis using 16569-1-AP (same clone as 16569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16569-1-AP (PPP2R2A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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  WB analysis using 16569-1-AP (same clone as 16569-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 16569-1-AP (PPP2R2A antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16569-1-AP (same clone as 16569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16569-1-AP (PPP2R2A antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 16569-1-AP (same clone as 16569-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 16569-1-AP (PPP2R2A antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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  IF Staining of A431 using 16569-1-AP (same clone as 16569-1-PBS)

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using PPP2R2A antibody (16569-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). This data was developed using the same antibody clone with 16569-1-PBS in a different storage buffer formulation.

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Informations sur le produit

16569-1-PBS cible PPP2R2A dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: PPP2R2A Protéine recombinante Ag9851
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit B, alpha isoform
• Masse moléculaire calculée: 477 aa, 52 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC041071
• Symbole du gène: PPP2R2A
• Identification du gène (NCBI): 5520
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The serine/threonine protein phosphatase 2A (PP2A) is a master regulator of the complex cellular signaling that occurs during all stages of mammalian development. PPP2R2A, also known as B55A or PR55A, is a regulatory subunit of PP2A expressed in an Akt-dependent manner in epidermis during barrier formation. PPP2R2A participates in the negative control of cell growth and division. PPP2R2A has been proved to be a tumor suppressor that can inhibit the proliferation of a variety of cancer cells, such as prostate cancer cells(PMID: 21872824).

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