Anticorps Monoclonal anti-PPP2R2A/B/C

PPP2R2A/B/C Monoclonal Antibody for WB, IHC, IF/ICC, Indirect ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, Indirect ELISA

Conjugaison

Non conjugué

CloneNo.

2E1D5

N° de cat : 67783-1-PBS

Synonymes

PPP2R2B, PP2A subunit B isoform B55-beta, PP2A PR55B, PP2A B55BETA, B55β

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Galerie de données de validation

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  WB analysis using 67783-1-Ig (same clone as 67783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours. The membrane was stripped and reblotted with HRP-conjugated GAPDH Monoclonal antibody (HRP-60004) as loading control. This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  WB analysis using 67783-1-Ig (same clone as 67783-1-PBS)

  Various lysates were subjected to SDS PAGE followed by western blot with 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours. This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse brain using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:300 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IHC staining of mouse cerebellum using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunohistochemical analysis of paraffin-embedded mouse cerebellum tissue slide using 67783-1-Ig (PPP2R2A/B/C antibody) at dilution of 1:300 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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  IF Staining of SH-SY5Y using 67783-1-Ig (same clone as 67783-1-PBS)

  Immunofluorescent analysis of (-20°C Ethanol) fixed SH-SY5Y cells using PPP2R2A/B/C antibody (67783-1-Ig, Clone: 2E1D5 ) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1). This data was developed using the same antibody clone with 67783-1-PBS in a different storage buffer formulation.

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Informations sur le produit

67783-1-PBS cible PPP2R2A/B/C dans les applications de WB, IHC, IF/ICC, Indirect ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: PPP2R2A/B/C Protéine recombinante Ag30774
• Nom complet: protein phosphatase 2 (formerly 2A), regulatory subunit B, beta isoform
• Masse moléculaire calculée: 443 aa, 52 kDa
• Poids moléculaire observé: 52 kDa
• Numéro d’acquisition GenBank: BC031790
• Symbole du gène: PPP2R2B
• Identification du gène (NCBI): 5521
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS only
• Conditions de stockage: Store at -80°C. 20ul contiennent 0,1% de BSA.

Informations générales

The PPP2R2B gene encodes a deduced 443 amino acid protein of approximately 52 kDa, which is a brain-specific regulatory subunit B of protein phosphatase 2. PPP2R2B is a Subunit of PP2A, a highly conserved constitutive enzyme(PMID:11719278). PPP2R2B (Bβ) is an important regulator of protein phosphatase 2A (PP2A) activity in the brain. Through differential promoter usage and alternative splicing, two major isoforms Bβ1 and Bβ2 with divergent sub-cellular targeting N termini are produced. Bβ plays an important role in neuronal survival. It has 5 isoforms produced by alternative splicing. This antibody can recognize PPP2R2A and PPP2R2C due to the immunogen of PPP2R2B having high homology with PPP2R2A and PPP2R2C.

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